Liofilchem™ Kanamycin/Polymyxin B Supplement

The Liofilchem™ Kanamycin/Polymyxin B Supplement is a selective additive designed to enhance the isolation of Clostridium perfringens. When added to Clostridium perfringens Agar and supplemented with Egg Yolk Emulsion, this supplement inhibits the growth of non-target organisms, ensuring reliable detection and enumeration of C. perfringens in clinical, food, and environmental samples.

Key Features

1. Selective for C. perfringens:
   • Contains Kanamycin (25 mg) and Polymyxin B (15,000 IU), which inhibit competing Gram-positive and Gram-negative bacteria.
2. Ready-to-Reconstitute:
   • Each vial is pre-measured for ease of use and reconstitution with 5 mL of sterile distilled water.
3. Flexible Applications:
   • Designed for use with Clostridium perfringens Agar (Codes 610207, 620207) and Egg Yolk Emulsion (Codes 80019, 80219).
4. Reliable Performance:
   • Validated with standard methods for detecting Clostridium perfringens.

Specifications

Preparation Instructions

1. Reconstitution:
   • Aseptically reconstitute the contents of one vial with 5 mL of sterile distilled water.
   • Mix until completely dissolved.
2. Medium Preparation:
   • Add the reconstituted supplement aseptically to 450 mL of Clostridium perfringens Agar (Code 610207 or 620207).
   • Ensure the medium is autoclaved, cooled to 50°C, and supplemented with 50 mL of Egg Yolk Emulsion (Code 80019 or 80219).
3. Mix:
   • Stir thoroughly to ensure even distribution and pour into sterile Petri dishes.

Applications

1. Clinical Microbiology:
   • Detection and enumeration of C. perfringens from clinical specimens such as feces and wound exudates.
2. Food Microbiology:
   • Identification of C. perfringens contamination in raw and processed food samples.
3. Environmental Testing:
   • Analysis of water and environmental samples for contamination with C. perfringens.

Advantages

• Highly Selective: Enhances recovery of Clostridium perfringens while suppressing background flora.
• Convenient: Ready-to-reconstitute lyophilized vials streamline preparation.
• Flexible Use: Compatible with a variety of applications in food, clinical, and environmental microbiology.
• ISO-Compliant: Supports compliance with international standards for C. perfringens detection.

Warnings and Precautions

1. For professional use only.
2. Handle all microbiological materials as potentially infectious.
3. Read all instructions carefully to ensure accurate and reliable results.
4. Consult the Safety Data Sheet (SDS) for safe handling and disposal guidelines.

Packaging

References

1. Harmon, S.M., Kautter, D.A., and Peeler, J.T. (1971). Improved medium for enumeration of Clostridium perfringens. App. Microbiol. 22:688.
2. Haushild A.H.W., and Hilsheimer, R. (1974). Evaluation and modifications of media for enumeration of C. perfringens. App. Microbiol. 27:78.
3. ICMSF (1978). Microorganisms in Foods: Their Significance and Methods of Enumeration. University of Toronto Press.
4. Shahidi, S.A., and Ferguson, A.R. (1971). New quantitative, qualitative, and confirmatory media for rapid analysis of food for C. perfringens. App. Microbiol. 21:500-506.

Why Choose Liofilchem Kanamycin/Polymyxin B Supplement?

• Trusted for reliable C. perfringens detection and enumeration.
• Easy-to-use format ensures consistent results in microbiological testing.
• Backed by Liofilchem’s expertise in producing high-quality microbiological products.