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Liofilchem™ Egg Yolk Tellurite Emulsion 20%, 4 x 50 mL
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Liofilchem | ||
| Product Grade | Microbiology grade | ||
ISO 6888-1 Compliant Supplement for Detection of Coagulase-Positive Staphylococcus aureus
Liofilchem® Egg Yolk Tellurite Emulsion 20% is a sterile, stabilized liquid supplement used in conjunction with Baird Parker Agar Base (Ref. 610004, 620004) for the isolation and enumeration of coagulase-positive staphylococci, including Staphylococcus aureus, in food and related samples. The complete medium, when prepared with this supplement, complies with ISO 6888-1 standards for microbial food safety analysis.
This supplement combines egg yolk (lecithin source) and potassium tellurite, which together allow for selective and differential growth of S. aureus based on lecithinase activity (clear halo around colonies) and tellurite reduction (black or grey colony pigmentation).
Key Features & Benefits
- Compliant with ISO 6888-1 – Supports validated microbiological food safety protocols
- Dual Detection Capability – Identifies S. aureus via lecithinase and tellurite reduction
- Ready-to-Use – Each 50 mL bottle supplements 1 liter of Baird Parker Agar
- Sterile & Stabilized Emulsion – Ensures consistent performance and extended shelf life
- Rapid Preparation – Requires only aseptic mixing with cooled, sterilized agar base
Composition (Per Bottle / Per Liter of Medium)
| Component | Per Bottle (50 mL) | Per Liter of Medium |
|---|---|---|
| Egg Yolk | 10 mL | 200 mL |
| Potassium Tellurite | 0.1 g | 2.0 g |
| Sterile Saline (0.9% NaCl) | 40 mL | 800 mL |
Intended Use
For the isolation, differentiation, and enumeration of coagulase-positive Staphylococcus aureus from food and animal feed samples using Baird Parker Agar, according to ISO 6888-1.
Preparation Instructions
- Autoclave 1 L of Baird Parker Agar Base and allow to cool to 45–50°C.
- Aseptically add one 50 mL bottle of Egg Yolk Tellurite Emulsion 20%.
- Mix thoroughly and pour into sterile Petri dishes.
- Inoculate and incubate at 37 ± 1°C for 24–48 hours.
Interpretation of Results (on Baird Parker Agar)
| Microorganism | Result |
|---|---|
| Staphylococcus aureus WDCM 00034 | Good growth; black/grey colonies with clear halo |
| Escherichia coli WDCM 00013 | Inhibited |
| Staphylococcus saprophyticus WDCM 00159 | Black/grey colonies without halo |
| Staphylococcus epidermidis WDCM 00036 | Black/grey colonies without halo |
Product Information
| Product Name | Reference Code | Packaging |
|---|---|---|
| Egg Yolk Tellurite Emulsion 20% | 80123 | 4 × 50 mL bottles |
One bottle is sufficient to prepare 1 liter of medium
Appearance & Storage
- Appearance: Yellow, opaque liquid. May contain resuspendable precipitate
- Storage: 2–8 °C, protected from light
- Stability: Valid for 1 year from date of manufacture
- Short-Term Tolerance: Stable for 4 days at 18–25 °C or 48 hours at 35–39 °C during transport
Warnings & Precautions
- Product is non-hazardous under applicable legislation
- For professional laboratory use only
- Use by trained personnel according to biosafety guidelines
- Do not use after expiration or if contamination is suspected
- Refer to the Safety Data Sheet (SDS) before use
Applications
- Detection of coagulase-positive staphylococci in food
- Microbiological quality control of dairy, meat, and ready-to-eat products
- Compliance with ISO 6888-1 protocols for S. aureus monitoring
- Academic research and routine food safety testing
The Liofilchem® Egg Yolk Tellurite Emulsion 20% is a critical supplement for the selective and differential enumeration of Staphylococcus aureus in compliance with ISO 6888-1. Its ready-to-use format, consistent performance, and ease of integration with Baird Parker Agar make it a reliable solution for food safety laboratories, regulatory testing facilities, and microbiology research environments.