Liofilchem Sodium selenite

Liofilchem Sodium Selenite is a highly selective reagent used in microbiology for the preparation of culture media, particularly selenite-based enrichment broths. It is primarily utilized for the isolation and selective enrichment of Salmonella spp. from fecal, food, and environmental samples. Sodium selenite inhibits the growth of competing organisms, allowing for better recovery of target pathogens.

Product Features

1. Selective Reagent:
   • Provides selective inhibition of non-Salmonella organisms, enabling effective recovery of Salmonella spp..
   • Especially useful in complex samples with high microbial loads, such as feces and food products.
2. Compatibility:
   • Designed for use with media such as Selenite Broth Base to prepare selenite-based enrichment media.
   • Works well in clinical, food, and environmental microbiology.
3. High Purity:
   • Formulated to meet the performance and quality requirements for microbiological applications.

Applications

1. Clinical Microbiology:
   • Enrichment of Salmonella spp. from fecal samples in diagnostic laboratories.
2. Food Microbiology:
   • Detects Salmonella spp. in food products, ensuring food safety and compliance.
3. Environmental Microbiology:
   • Used to recover Salmonella spp. from water, sewage, and other environmental sources.

Specifications

Preparation Instructions

1. Solution Preparation:
   • Dissolve 4 g of sodium selenite in 1 L of distilled water to prepare a concentrated solution.
2. Media Preparation:
   • Add the sodium selenite solution to a suitable basal medium, such as Selenite Broth Base, as per the medium preparation protocol.
3. Sterilization:
   • Sterilize in a boiling water bath or free-flowing steam for 10 minutes.
   • Avoid autoclaving, as high temperatures degrade selenite and reduce its selectivity.

Safety Precautions

1. Toxicity:
   • Sodium selenite is toxic by inhalation or ingestion.
   • Handle with caution, using appropriate personal protective equipment (PPE).
   • Ensure proper ventilation during handling and preparation.
2. Regulatory Compliance:
   • Follow local and national guidelines for handling and disposal.
3. Health Risks:
   • Inhalation or ingestion may cause adverse health effects. Avoid exposure during preparation.

Storage and Stability

• Storage Conditions:
  • Store at 10–30°C in a cool, dry place, in its original sealed container.
• Shelf Life:
  • Check the expiry date on the product label; do not use after expiration.
• Prepared Media:
  • Store prepared media at 2–8°C and protect from light.

Advantages

1. High Selectivity:
   • Effectively inhibits non-target organisms in complex samples.
2. Wide Applications:
   • Suitable for clinical, food, and environmental microbiology.
3. Cost-Effective:
   • Facilitates reliable isolation of Salmonella spp., reducing false negatives.

Applications Summary

1. Enrichment Media:
   • Critical for the preparation of selenite-based enrichment broths, which are widely used for Salmonella detection.
2. Sample Processing:
   • Ideal for complex matrices like feces, food products, and water samples.
3. Laboratory Use:
   • Supports workflows in diagnostic, food safety, and environmental testing laboratories.

Liofilchem Sodium Selenite is a vital component for laboratories conducting selective enrichment and recovery of Salmonella spp., ensuring reliable and reproducible results in microbiological testing. Let me know if you need additional details or usage tips!