Sigma-Aldrich Calbiochem® TMPyP4 ≥90% (TLC), telomerase inhibitor, 25mg

High-Purity TMPyP4 for Telomerase Inhibition & DNA Quadruplex Research

TMPyP4 (5,10,15,20-Tetrakis(1-methyl-4-pyridyl)porphyrin) is a potent inhibitor of human telomerase (IC₅₀ = 6.5 µM). It binds strongly to G-quadruplex DNA structures, stabilizing them and leading to the inhibition of telomerase activity. This compound is widely used in cancer research, molecular biology, and nucleic acid studies to investigate quadruplex-mediated gene regulation and telomere function.

Key Features & Benefits

✅ Highly Pure & Stable

• ≥90% purity (TLC) for reproducible research results.
• Stable at 2-8°C, desiccated, and protected from light to prevent degradation.

✅ Potent Telomerase Inhibitor

• IC₅₀ = 6.5 µM in human telomerase inhibition assays.
• Binds G-quadruplex DNA, stabilizing the structure and blocking telomerase activity.

✅ DNA Quadruplex Fluorescent Binder

• Strong fluorescence enhancement in the presence of quadruplex DNA, making it useful for quadruplex-binding studies.

✅ Suitable for Cancer & Molecular Biology Research

• Investigates telomerase inhibition as a potential anticancer strategy.
• Used to study quadruplex-mediated gene regulation in oncogenic pathways.

✅ Cited in Multiple Research Studies

• Referenced in Cancer Research, Nucleic Acids Research, J. Am. Chem. Soc., and other peer-reviewed journals.

Technical Specifications

Biological & Chemical Actions

• Binds strongly to G-quadruplex DNA, stabilizing G-tetrads at the quadruplex core.
• Inhibits human telomerase activity by blocking telomere elongation, leading to potential anticancer effects.
• Fluorescent properties allow tracking of quadruplex formation and interactions in molecular biology studies.

Use Cases & Applications

1. Cancer Research & Telomerase Inhibition Studies

Primary Use:

• Investigates telomerase inhibition as a potential anticancer therapy.
• Used in telomere-related drug discovery and screening.

Practical Example:

A cancer research lab uses TMPyP4 to study telomere shortening effects in tumor cells, evaluating its potential for anticancer drug development.

2. DNA Quadruplex Studies & Gene Regulation

Primary Use:

• Used in quadruplex-targeting studies to explore gene regulation mechanisms.
• Helps assess quadruplex interactions with transcription factors and small molecules.

Practical Example:

A molecular biology team studies quadruplex formation in oncogene promoters (e.g., c-MYC, KRAS) using TMPyP4 to assess transcriptional repression mechanisms.

3. Fluorescent DNA Binding & Molecular Imaging

Primary Use:

• Fluoresces intensely when bound to G-quadruplex DNA, making it useful for fluorescence-based assays.
• Tracks quadruplex formation in live-cell imaging and in vitro assays.

Practical Example:

A biophysics lab uses TMPyP4 in fluorescence resonance energy transfer (FRET) assays to monitor quadruplex formation dynamics under physiological conditions.

4. Drug Discovery & High-Throughput Screening

Primary Use:

• Acts as a positive control in quadruplex-stabilizing compound screening.
• Evaluates small-molecule inhibitors of telomerase and quadruplex-interacting proteins.

Practical Example:

A pharmaceutical R&D team screens quadruplex-binding compounds using TMPyP4 as a reference inhibitor to compare novel drug candidates.

References & Supporting Studies

• Yamashita, T., et al. (2005) – Bioorg. Med. Chem. 13, 2433.
• Izbicka, E., et al. (1999) – Cancer Res. 59, 639.
• Anantha, N.V., et al. (1998) – Biochemistry 37, 2709.
• Arthanari, H., et al. (1998) – Nucleic Acids Res. 26, 3724.
• Wheelhouse, R.T., et al. (1998) – J. Am. Chem. Soc. 120, 3261.

Why Choose Sigma-Aldrich Calbiochem® TMPyP4?

🧬 Proven Telomerase Inhibitor – IC₅₀ = 6.5 µM for telomerase inhibition.

🔬 Ideal for Cancer Research & Gene Regulation Studies – Investigates quadruplex interactions.

💡 Fluorescent DNA Binder – Enhances quadruplex detection in molecular biology assays.

⚡ High Purity (≥90%) & Reliable Performance – Ensures reproducibility in experimental research.

🛡 Stable Storage & Easy Handling – Can be stored at 2-8°C, desiccated, and protected from light.

The Sigma-Aldrich Calbiochem® TMPyP4 (≥90% TLC, 25mg) is a high-quality, research-grade inhibitor of telomerase and G-quadruplex stabilizer. It is a valuable tool for cancer research, gene regulation studies, and quadruplex-targeting drug discovery. With fluorescence properties and high binding affinity, TMPyP4 is widely used in biophysics, molecular biology, and pharmaceutical development.