The Liofilchem® Malt Extract Broth is a liquid medium specially designed for the cultivation of yeasts and moulds. Its formulation provides essential nutrients and an acidic pH to support fungal growth while inhibiting bacterial proliferation.
Key Features
- Optimized for Yeasts and Moulds:
- Acidic pH ensures robust fungal growth and minimizes bacterial contamination.
- Nutrient-Rich Formula:
- Includes malt extract, glucose, and yeast extract for enhanced organism growth.
- Easy Preparation:
- Dehydrated powder allows quick and consistent preparation of the medium.
- Wide Application:
- Suitable for food, pharmaceutical, and environmental fungal analyses.
Specifications
Formula
Component | Amount (g/L) |
---|
Malt Extract | 6.0 |
Maltose | 1.8 |
Glucose | 6.0 |
Yeast Extract | 1.2 |
Final pH | 4.7 ± 0.2 |
Physical Properties
Parameter | Details |
---|
Dehydrated Medium | Free-flowing, homogeneous, beige |
Prepared Medium | Clear, light amber |
Environmental and Storage
Condition | Details |
---|
Storage Temperature | 10-30°C (Dehydrated Medium) |
| 2-8°C (Prepared Medium) |
Shelf Life | 4 years |
Packaging Options
Reference | Content | Packaging |
---|
610069 | 500 g | Plastic Bottle |
620069 | 100 g | Plastic Bottle |
Applications
- Food Safety Testing:
- Used to detect fungal contamination in food and beverage products.
- Pharmaceutical Testing:
- Ensures safety and quality in pharmaceutical environments.
- Environmental Monitoring:
- Monitors fungal growth in environmental samples.
Usage Guidelines
Preparation
- Suspend 15 g of powder in 1 liter of distilled or deionized water.
- Heat and stir until fully dissolved.
- Dispense into final containers.
- Sterilize at 121°C for 15 minutes.
Technique
- Inoculate the medium with the test organism.
- Incubate at 30 ± 2°C for 18-72 hours under aerobic conditions.
- Observe for turbidity, indicating fungal growth.
Microbial Performance
Microorganism | ATCC® | Growth |
---|
Aspergillus brasiliensis | 16404 | Good |
Candida albicans | 10231 | Good |
Saccharomyces cerevisiae | 9763 | Good |
Advantages
- Reliable Results:
- Provides consistent fungal growth with minimized bacterial interference.
- Comprehensive Nutrients:
- Supports a wide variety of fungal species.
- Long Shelf Life:
- Stable for 4 years when stored correctly.
- Regulatory Compliance:
- Meets industry standards for fungal detection.
Warnings and Precautions
- Use only by trained personnel in a laboratory setting.
- Store in a dry environment to avoid contamination.
- Dispose of waste in accordance with local and national regulations.
References
- Reddish. (1919). Abstr. Bacteriol.
- Thom and Church. (1926). The Aspergilli.
- U.S. Food and Drug Administration (2001). Bacteriological Analytical Manual, AOAC International.
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