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BD™ CBA Human Angiogenin Flex Set, 100 Tests
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | BD Biosciences | ||
| Product Grade | Molecular Biology | ||
High-Precision Flow Cytometry Assay for Angiogenin Detection in Human Samples
The BD™ CBA Human Angiogenin Flex Set (Catalog No. 558328) is a specialized bead-based immunoassay designed for the quantitative measurement of human Angiogenin in serum, EDTA-treated plasma, and cell culture supernatant samples. Utilizing the BD CBA Flex Set platform, this reagent set delivers sensitive and reproducible detection of angiogenin, a multifunctional protein involved in angiogenesis, tumor progression, and immune modulation.
With an assay range of 10–2,500 pg/mL and a limit of detection of 4.6 pg/mL, the kit offers a powerful tool for basic and translational research. For optimal performance, this set must be used in conjunction with the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265) and a flow cytometer equipped with compatible software such as FCAP Array™.
Key Features
Specific Target Detection: Measures human Angiogenin concentrations in multiple biological matrices
Multiplex-Ready Bead Format: Uses fluorescent bead position C4; do not multiplex with other C4-positioned Flex Sets
High Sensitivity: Detects Angiogenin as low as 4.6 pg/mL, with a quantification range of 10–2,500 pg/mL
Flow Cytometry-Based Assay: Compatible with BD FACSCanto™, BD FACSArray™, and other standard flow cytometers
RUO-Labeled: For Research Use Only, not intended for clinical diagnostics
Standard Curve Included: Includes lyophilized standard for accurate quantitative analysis
Validated Sample Types: Use with 1:4 diluted human serum, plasma, and culture supernatants
Applications
- Quantification of Angiogenin in cancer or tumor microenvironment studies
- Angiogenesis and vascular biology research
- Biomarker detection in inflammation and immune regulation
- Evaluation of angiogenin expression in cell culture supernatants
- Translational research in cardiovascular and neurological disorders
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | BD™ CBA Human Angiogenin Flex Set |
| Catalog Number | 558328 |
| Assay Type | Bead-based immunoassay |
| Detection Method | Flow cytometry with PE detection reagent |
| Analyte | Human Angiogenin |
| Assay Range | 10–2,500 pg/mL |
| Limit of Detection | 4.6 pg/mL |
| Sample Types | Human serum, EDTA plasma, cell culture supernatant |
| Bead Position | C4 |
| Reactivity | Human (QC Tested) |
| Number of Tests | 100 |
| Regulatory Status | RUO – Research Use Only |
| RRID | AB_2869158 |
| Required Kit | BD CBA Master Buffer Kit (Cat. No. 558264 or 558265) |
| Storage | 2–8 °C (protect from light; do not freeze) |
Kit Components
| Component | Part Number | Quantity |
|---|---|---|
| Human Angiogenin Capture Bead (C4) | 51-9005333 | 100 Tests |
| Human Angiogenin PE Detection Reagent | 51-9004219 | 100 Tests |
| Human Angiogenin Standard (lyophilized) | 51-9004216 | 2 vials |
The BD™ Human Angiogenin Flex Set is a reliable, high-sensitivity tool for detecting Angiogenin levels in biological samples using flow cytometry. Its precision, compatibility with BD CBA systems, and flexibility for multiplexing make it ideal for angiogenesis research, tumor biology, and biomarker validation in complex sample matrices.