KingFisher™ Duo Prime Purification System

The Thermo Scientific™ KingFisher™ Duo Prime Purification System is a compact, benchtop magnetic particle processor ideal for low-to-medium throughput nucleic acid, protein, and cell purification workflows. Designed to deliver high-quality, reproducible results with minimal hands-on time, the Duo Prime is perfect for labs performing routine or customized extraction protocols from a wide variety of sample types.

Its flexible design accommodates volumes up to 5 mL, supports both DNA/RNA isolation and proteomics, and features automated magnetic bead technology for efficient purification. Additional upgrades such as a built-in UV decontamination lamp and optional barcode reader enhance contamination control and sample traceability.

✅ Key Features & Benefits

🧲 Automated Magnetic Bead Technology

• Utilizes Thermo Fisher’s proven magnetic particle processing, ensuring ≥95% particle collection efficiency.
• Delivers high recovery rates of nucleic acids, proteins, or cells across a wide range of input types.

🔄 Flexible Throughput & Sample Types

• Up to 12 samples per run (12-pin head) or 6 samples (6-pin head).
• Suitable for processing body fluids, blood, bacteria, cell cultures, tissues, and plants.
• Supports elution volumes of 30–130 µL and processing volumes up to 5 mL.

💡 Smart Lab Features

• 8W UV lamp enables easy decontamination between runs (exposure up to 16 hrs).
• Optional barcode reader for seamless sample tracking and traceability.

🧠 Advanced Protocol Control with BindIt™ Software

• Create, modify, and save up to 200 protocols.
• Import methods via PC or USB stick.
• Automatic run log file saving for compliance and traceability.

🌡️ Precision Heating & Cooling

• Heating range: 10°C to 75°C (plate row A).
• Cooling range: 4°C to 75°C (elution strip).
• Facilitates optimal elution and lysis conditions.

⚙️ Specifications

🧪 Recommended Applications

• DNA and RNA purification from:
  • Blood, plasma, serum, buccal swabs, FFPE tissue, plants, etc.
• Protein purification and enrichment (e.g., immunoprecipitation)
• Cell isolation and pathogen enrichment
• Veterinary diagnostics
• Forensic sample processing
• Custom assay development and pilot studies

🧬 Use Case Examples

🔬 Academic and R&D Labs

• Optimize protocols with easy-to-edit BindIt software.
• Ideal for labs running limited batches or performing pilot testing.

🧫 Clinical Research & Forensics

• Use UV decontamination to avoid cross-contamination in sensitive applications.
• Perfect for low-volume high-value samples (e.g., forensic samples).

🧪 Biotech & Pharma Labs

• Suitable for biomarker discovery, proteomics, and gene expression workflows.
• Compact design fits easily into molecular biology benches.

🔍 Comparison: KingFisher Duo Prime vs. KingFisher Flex

✅ Why Choose the KingFisher™ Duo Prime?

• Compact and budget-friendly system for labs with limited throughput needs
• Ideal for pilot testing, R&D, teaching labs, and sensitive assays
• Built-in UV lamp for contamination-free operation
• Easy-to-use software with full protocol customization
• Supports a wide range of magnetic bead-based kits and chemistries