0
Thermo Scientific™ Applied Biosystems™ Tempus™ Blood RNA Tube
Catalog No :
CAS Number :
Brand :
In Stock
Each Tempus Blood RNA Tube contains 6 mL Stabilizing Reagent
Specifications:
| Application | Automated Nucleic Acid Purification, RNA Extraction |
| Product Type | Blood RNA Stabilization Tubes |
| Product Brand | Applied Biosystems™ |
| Product Grade | Molecular Biology |
The Tempus™ Blood RNA Tubes are specifically designed for the collection, stabilization, and isolation of high-quality RNA from whole blood, providing an efficient solution for gene expression analysis. These tubes allow for the immediate lysis of blood cells upon collection, ensuring RNA integrity and stability for downstream applications.
Key Features
- Immediate RNA Stabilization:
- Blood cells are lysed upon collection, stabilizing RNA and preventing degradation.
- No need for additional red blood cell lysis or proteinase K treatments.
- Long-Term Stability:
- RNA remains stable for up to 5 days at room temperature or 7 days at 4°C.
- Streamlined Workflow:
- Simplifies sample handling and storage with stabilization in a single step.
- Accessory Compatibility:
- Works seamlessly with the Tempus Spin RNA Isolation Kit for enhanced RNA stability and higher yields.
Specifications
| Feature | Details |
|---|---|
| Description | Blood RNA Stabilization Tubes |
| Sample Type | Whole Blood |
| Capacity | 3 mL of whole blood with 6 mL stabilizing reagent |
| Storage Conditions | Room Temperature |
| Shipping Condition | Room Temperature |
| Shelf Life | Greater than 12 months |
| Unit Size | 50 tubes |
| Applications | RNA isolation for gene expression analysis |
| For Use With | Tempus Spin RNA Isolation Kit |
Kit Contents
- 50 Tempus Blood RNA Tubes, each containing:
- 6 mL Stabilizing Reagent for immediate lysis and RNA stabilization.
Advantages
- Efficiency: One-step RNA stabilization reduces handling time and risk of degradation.
- Stability: Maintains RNA integrity for multiple days without freezing.
- Compatibility: Pairs effectively with downstream RNA isolation kits, ensuring high yields and purity.
- Ease of Use: Simplifies workflows, especially in large-scale or multicenter studies.
Comparison with PAXgene™ Blood RNA Tubes
| Feature | Tempus™ Blood RNA Tubes | PAXgene™ Blood RNA Tubes |
|---|---|---|
| RNA Stabilization | Immediate with no additional treatments | Stabilizes RNA upon collection |
| Sample Stability | 5 days (RT), 7 days (4°C) | Up to 3 days (RT), longer at 4°C |
| Ease of Use | No RBC lysis or proteinase K treatments | Requires RBC lysis for certain protocols |
| Accessory Compatibility | Tempus Spin RNA Isolation Kit | PAXgene RNA Extraction Kit |
| Volume of Blood Collected | 3 mL | 2.5 mL |
| Storage of Tubes | Room Temperature | Room Temperature |
| RNA Yield and Integrity | High yields with minimal degradation | Comparable with optimized protocols |
Applications
- Gene Expression Profiling:
- Ideal for analyzing RNA biomarkers in clinical and research studies.
- RNA-based Studies:
- Suitable for transcriptomics, RT-PCR, and next-generation sequencing (NGS).
- Biomarker Discovery:
- Effective in identifying RNA-based biomarkers for disease studies.
- Multicenter Research:
- Long-term RNA stabilization simplifies logistics for multicenter studies.
Research Citations
- Matheson et al. (2008): Assessment of sample collection and storage methods for multicenter immunologic research in children - PubMed ID: 18771669
- Menke et al. (2012): Peripheral blood gene expression: it all boils down to the RNA collection tubes - PubMed ID: 22214347
- Asare et al. (2008): Differential gene expression profiles are dependent upon method of peripheral blood collection and RNA isolation - PubMed ID: 18847473
- Rollins et al. (2010): Analysis of whole genome biomarker expression in blood and brain - PubMed ID: 20127885
- Berry et al. (2010): An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis - PubMed ID: 20725040
The Tempus™ Blood RNA Tubes offer unparalleled efficiency and RNA stabilization, making them an ideal choice for clinical and research applications involving gene expression and biomarker studies.