Sigma-Aldrich α-Cyano-4-hydroxycinnamic acid matrix substance for MALDI-MS, Ultra pure, 10x10mg

Sigma-Aldrich α-Cyano-4-hydroxycinnamic acid (α-CHCA) is an ultra-pure matrix substance specifically designed for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.

• As a matrix, α-CHCA aids in the efficient ionization of proteins, peptides, and other analytes for accurate detection in MALDI-TOF (time-of-flight) mass spectrometers.
• Packaged in 10 vials of 10mg each, this ultra-pure formulation ensures minimal contamination and maximized reproducibility in analytical workflows.

Key Features:

• Ultra-pure grade: Ensures precise and consistent results by minimizing background noise in mass spectra.
• Matrix-specific for MALDI: Facilitates the desorption and ionization process essential for MALDI-MS applications.
• Effective for low-concentration samples: Enhances the detection sensitivity of low-abundance proteins and peptides.

Specifications:

• Chemical Name: α-Cyano-4-hydroxycinnamic acid
• Chemical Formula: C10H7NO3
• Molecular Weight: 189.17 g/mol
• Purity: Ultra-pure grade
• Packaging: 10 x 10mg vials

Applications:

1. Proteomics:
   • Effective in the ionization of peptides and proteins for detailed mass spectrometry analysis.
   • Commonly used in bottom-up proteomics workflows to identify protein digests.
2. Biomarker Discovery:
   • Applied in clinical research for detecting low-concentration biomarkers in complex biological samples.
3. Peptide Mapping:
   • Facilitates high-resolution peptide mapping for protein characterization and identification.
4. Metabolomics:
   • Useful in metabolite analysis, especially for small molecules requiring MALDI ionization.
5. Drug Discovery:
   • Supports the identification and characterization of small drug molecules in pharmaceutical research.
6. High Sensitivity Assays:
   • Enhances the performance of MALDI-MS assays for trace-level analyte detection.

Benefits in MALDI-MS:

• High Signal Intensity: Produces strong analyte signals with reduced matrix-related interference.
• Optimized Desorption: Facilitates efficient energy transfer during the laser desorption process.
• Versatile Compatibility: Suitable for a wide range of analytes, including peptides, proteins, and small molecules.

Storage and Handling:

• Store in a cool, dry place, away from light and moisture.
• Use with appropriate safety measures, including gloves and eye protection, as α-CHCA is sensitive to environmental conditions.

Sigma-Aldrich α-Cyano-4-hydroxycinnamic acid (α-CHCA) is an essential matrix for MALDI-MS workflows, delivering unparalleled purity and performance for proteomics, biomarker discovery, and other advanced applications in analytical sciences. Its ability to enhance signal intensity and detect low-concentration analytes makes it an indispensable tool for researchers working with MALDI-TOF MS.