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Thermo Scientific™ Blocker™ blocking buffer BSA (10X) in PBS, 200 mL
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
High-Purity Bovine Serum Albumin Blocking Reagent for Western Blot, ELISA, IHC, and Nucleic Acid Assays
Thermo Scientific™ Blocker™ BSA is a ready-to-use 10% (w/v) solution of purified bovine serum albumin (BSA) formulated in phosphate-buffered saline (PBS). It is specifically designed to block nonspecific binding sites on membranes, microplates, and tissue sections in immunoassays such as Western blotting, ELISA, immunohistochemistry (IHC), and nucleic acid detection protocols.
Compared to nonfat milk, Blocker BSA offers higher consistency and reduced background, particularly in biotin-avidin detection systems, due to the absence of endogenous biotin. This makes it the blocking agent of choice for sensitive and specific immunodetection workflows.
Key Features & Benefits
- Purified Single-Protein Blocking Agent – Minimizes background by eliminating interfering proteins
- High Concentration Format – Supplied at 10% (w/v) and typically diluted 1:10 for working use (1% final concentration)
- PBS-Buffered – Ready to use in workflows requiring phosphate-buffered saline conditions
- Enhanced Signal Clarity – Reduces nonspecific binding for improved assay reproducibility
- Biotin-Free – Ideal for biotin-avidin/streptavidin detection systems
- Convenient Packaging – Supplied as 3 × 200 mL bottles for extended usability in high-throughput labs
Applications
- Western Blotting – Block membranes before primary antibody incubation
- ELISA (Enzyme-Linked Immunosorbent Assay) – Coat microplates to prevent nonspecific analyte adsorption
- Immunohistochemistry (IHC) – Block tissue sections for reduced background staining
- Nucleic Acid Detection – Use in hybridization protocols to minimize probe binding to uncoated surfaces
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | Blocker™ BSA, 10% Solution |
| Concentration | 10% BSA (w/v) |
| Form | Liquid |
| Blocking Agent | Single purified protein (bovine serum albumin) |
| Recommended Working Dilution | 1:10 (1% final concentration) |
| Product Line | Thermo Scientific™ Blocker™ |
| For Use With | Western Blot, ELISA, IHC, Nucleic Acid Detection |
| Storage Conditions | Store at 4°C |
| Regulatory Use | For Research Use Only |
Blocking Buffer Selection Guide
| Use Case | Recommended Buffer | Available Formats | Why Choose This Buffer |
|---|---|---|---|
| Need a versatile blocker for new or routine western blot optimization | StartingBlock™ Blocking Buffer | PBS, PBST, TBS, TBST | Performs well with a broad range of antibodies and combinations; ideal for general use |
| Seeing high background or poor reproducibility with milk-based buffers | Blocker™ Casein | PBS, TBS | Offers reduced cross-reactivity; consistent performance over serum/milk blocking agents |
| Performing fluorescent western blots or using fluorescent detection | Blocker™ FL Fluorescent Buffer | 10X concentrate | Detergent-free; minimizes background fluorescence in sensitive fluorescence assays |
| Targeting phospho-proteins or storing antibodies in buffer long-term | Blocker™ BSA | 10X concentrate | Biotin-free, purified BSA for minimal cross-reaction with sensitive targets or assay steps |
Quick Tips
- Choose Blocker™ BSA for biotin-avidin systems, phospho-protein work, or when extended storage is required.
- Use Blocker™ FL for fluorescence-based detection to prevent detergent-related interference.
- Switch to Blocker™ Casein if experiencing high background or batch variability.
- StartingBlock™ is your go-to buffer when setting up or running standard western blot protocols.
Thermo Scientific™ Blocker™ BSA (10% in PBS) is a reliable, consistent, and biotin-free blocking solution ideal for high-sensitivity immunodetection workflows. Whether used in Western blots, ELISAs, or IHC, its high purity and compatibility with biotin-based systems make it the preferred choice for reducing background noise and improving assay accuracy.
- Pack Size: 200 mL in PBS Buffer 3 X 200 mL in PBS Buffer 125 mL in TBS Buffer