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Invitrogen™ Integrin beta 7 Monoclonal Antibody (FIB504), PE-Cyanine7, eBioscience™, 100 Tests
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Integrin Beta 7 Monoclonal Antibody (FIB504), PE-Cyanine7, eBioscience™ (Catalog # 25-5867-42) is a highly specific flow cytometry antibody designed for the detection of integrin beta 7 in human and mouse samples. This PE-Cyanine7-conjugated monoclonal antibody enables high-sensitivity identification of integrin beta 7-expressing cells, which play a crucial role in mucosal immunity and lymphocyte adhesion.
Integrin beta 7 forms heterodimers with integrin alpha 4 (CD49d) and alpha E (CD103) to mediate leukocyte trafficking and adhesion, particularly within the gastrointestinal and respiratory mucosa. This antibody is validated for flow cytometry applications, providing clear and reproducible staining results for immunology and inflammation research.
Key Features & Benefits
1. High-Specificity Detection of Integrin Beta 7
- Recognizes human and mouse integrin beta 7, allowing for cross-species applications.
- Targets integrin beta 7, which forms key heterodimers (LPAM-1 & HML-1) involved in leukocyte adhesion.
- Facilitates precise analysis of mucosal T cells, intraepithelial lymphocytes (IELs), and gut-homing immune cells.
2. PE-Cyanine7 Conjugation for High-Sensitivity Flow Cytometry
- Conjugated to PE-Cyanine7 (Excitation: 569 nm / Emission: 780 nm) for strong fluorescence signal.
- Compatible with blue, green, and yellow-green lasers, ensuring minimal spectral overlap.
- Provides robust and clear staining for multicolor flow cytometry panels.
3. Optimized for Flow Cytometry & Immunophenotyping
- Pre-titrated and tested for use at 5 µL (0.125 µg)/test, ensuring consistent and reproducible staining.
- Allows for accurate gating of lymphocyte subsets expressing integrin beta 7.
- Compatible with isotype controls and fluorescence compensation strategies.
4. Fixation-Compatible & Long-Term Storage Stability
- Fixation buffer compatible (IC Fixation Buffer, 1-Step Fix/Lyse Solution) for sample storage up to 3 days.
- Stable at 4°C in the dark, with photo-sensitive protection to prevent fluorophore degradation.
Product Specifications
| Property | Details |
|---|---|
| Brand | Invitrogen™ eBioscience™ |
| Antibody Target | Integrin Beta 7 |
| Clone | FIB504 |
| Isotype | Rat IgG2a, kappa |
| Species Reactivity | Human, Mouse |
| Conjugate | PE-Cyanine7 (PE-Cy7) |
| Excitation/Emission Max | 569/780 nm |
| Application | Flow Cytometry (FC) |
| Recommended Dilution | 5 µL (0.125 µg)/test |
| Form | Liquid |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA and 0.09% sodium azide |
| Storage Conditions | 4°C, store in the dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic), Wet Ice (international) |
| RRID | AB_2573481 |
Available Variants & Ordering Information
| Catalog Number | Conjugate | Unit Size |
|---|---|---|
| 25-5867-42 | PE-Cyanine7 | 100 Tests |
| 14-5867-82 | Unconjugated | 100 µg |
| 12-5867-42 | PE | 100 Tests |
| 11-5867-42 | FITC | 100 Tests |
| 13-5867-82 | Biotin | 100 Tests |
Use Cases & Applications
1. Immunophenotyping of Mucosal & Intraepithelial Lymphocytes
- Detection of gut-homing T cells expressing α4β7 and αEβ7 integrins.
- Analysis of T cells, B cells, and dendritic cells in mucosal immune responses.
2. Inflammation & Autoimmune Disease Research
- Investigating integrin-mediated leukocyte trafficking in inflammatory bowel disease (IBD).
- Examining the role of beta 7 integrin in chronic inflammation and immune homeostasis.
3. Tumor Immunology & Cancer Research
- Studying integrin beta 7-expressing T cells in tumor microenvironments.
- Evaluating the impact of integrin signaling on immune cell infiltration in cancer.
4. Infectious Disease & Vaccine Research
- Assessing immune cell migration to mucosal tissues following infection or vaccination.
- Studying integrin-mediated immune responses in respiratory and gastrointestinal infections.
Performance Data & Validation
1. High-Purity Staining in Flow Cytometry
- Tested on normal human peripheral blood cells, yielding strong, specific staining in the lymphocyte gate.
- Shown to distinguish integrin beta 7-expressing cells with minimal background signal.
2. Validated for Use in Multicolor Panels
- Compatible with APC-labeled T-cell markers for detailed immunophenotyping.
- Works alongside CD3, CD103, and VCAM-1 markers to study immune cell adhesion and migration.
Target Information: Integrin Beta 7 (ITGB7)
Integrin beta 7 is a glycoprotein subunit that forms key heterodimers involved in immune cell adhesion and migration. It associates with integrin alpha 4 (CD49d) to form LPAM-1 (α4β7) and with integrin alpha E (CD103) to form HML-1 (αEβ7). These integrins regulate lymphocyte trafficking, gut-homing of T cells, and immune responses in mucosal tissues.
Ligands & Functional Role:
- LPAM-1 (α4β7) binds to VCAM-1 (CD106), MAdCAM-1, and fibronectin to mediate leukocyte adhesion to endothelium.
- HML-1 (αEβ7) interacts with E-cadherin (CD324), supporting T-cell retention in epithelial layers.
- Essential for T-cell migration, immune surveillance, and inflammatory responses.
Aliases:
- ITGB7, Gut homing receptor beta subunit, M290 IEL antigen
UniProt ID:
- Human: P26010
- Mouse: P26011
The Invitrogen™ Integrin Beta 7 Monoclonal Antibody (FIB504), PE-Cyanine7 is a high-sensitivity flow cytometry antibody designed for detection of integrin beta 7 in immune cells. Featuring PE-Cyanine7 conjugation for strong fluorescence signal, high specificity for human and mouse integrin beta 7, and compatibility with multicolor flow cytometry panels, this antibody is ideal for studying immune cell migration, mucosal immunity, and leukocyte adhesion in inflammation and tumor microenvironments.