Gibco™ HBSS, calcium, magnesium

Gibco™ Hanks' Balanced Salt Solution (HBSS) with calcium, magnesium, glucose, and phenol red is a sterile, ready-to-use buffer solution designed for a wide range of cell culture applications. This formulation is ideal for cell washing, tissue transport, reagent preparation, and dilution of cells before counting or further processing.

The inclusion of calcium and magnesium supports cell adhesion, intercellular communication, and physiological ion balance, making this HBSS formulation particularly suitable for primary cell isolation and serum-free culture applications. The presence of glucose provides energy support for short-term cell maintenance, while phenol red serves as a pH indicator to facilitate monitoring of buffer conditions during use.

Key Features & Benefits

1. Optimized for Cell Culture & Tissue Transport
   • Maintains cell viability and osmotic balance during washing and transport.
   • Prevents shear stress and maintains adhesion in adherent cell cultures.
2. Calcium & Magnesium for Enhanced Cell Integrity
   • Supports cell attachment and intercellular communication.
   • Reduces disruption of cell-to-cell junctions in adherent cultures.
3. Glucose-Enriched for Metabolic Support
   • Provides a short-term energy source for cells during processing.
   • Helps reduce cell stress during washing and dilution procedures.
4. Phenol Red Indicator for pH Stability
   • Visually monitors pH changes in the buffer.
   • Ensures optimal physiological conditions for cell viability.
5. Sterile, Pre-Filtered, and cGMP-Compliant
   • Manufactured under ISO 13485 standards for high-quality research applications.
   • Ensures consistent experimental reproducibility and long-term stability (36 months shelf life).

Product Specifications

Use Cases & Applications

1. Cell Washing & Tissue Transport

• Prepares Cells for Downstream Experiments
  • Maintains osmotic balance and cell viability during processing.
  • Suitable for washing cells before media changes or cryopreservation.
• Ideal for Tissue Transport & Storage
  • Prevents osmotic shock and cell stress when transporting tissue samples.
  • Used in biopsies and primary cell research workflows.

2. Mammalian Cell Culture & Reagent Preparation

• Serum-Free & Custom Media Preparation
  • Works as a base solution for serum-free and chemically defined media.
  • Suitable for nutrient dilution and preparation of assay reagents.
• Optimized for Cell Attachment & Growth
  • Maintains cell-to-cell interactions in adherent cell cultures.
  • Enhances cell integrity during washing steps and enzymatic treatments.

3. Immunohistochemistry (IHC) & Functional Assays

• Supports Cell Fixation & Staining Protocols
  • Provides a stable, physiologically buffered environment for sample processing.
  • Reduces nonspecific cell lysis during antibody labeling procedures.
• Maintains Cell Functionality in Physiological Conditions
  • Ensures stable pH balance and electrolyte composition.
  • Ideal for preclinical drug screening and cytotoxicity assays.

4. Stem Cell & Organoid Research

• Maintains Cell Viability During Dissociation & Passaging
  • Prevents calcium-dependent junction breakdown during cell handling.
  • Enhances stem cell culture conditions by maintaining ion balance.
• Supports Neural & Cardiac Tissue Engineering
  • Ideal for cardiomyocyte and neural cell research.
  • Works in co-culture systems for tissue regeneration models.

Instructions for Use

General Handling & Preparation

1. Pre-Warm Before Use:
   • Warm HBSS to room temperature or 37°C before application.
2. For Cell Washing & Transport:
   • Gently resuspend cells or tissue in HBSS.
   • Centrifuge at 300-400 x g for 5 minutes, discard the supernatant, and proceed with culture.
3. For Serum-Free Culture & Assay Preparation:
   • Dilute supplements, growth factors, or media components as required.
   • Use as a base solution for custom formulations.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 15–30°C (Room Temperature).
  • Avoid prolonged light exposure to maintain stability.
• Handling Precautions:
  • Use aseptic techniques to prevent contamination.
  • Work in a biosafety cabinet (BSC) when handling sterile media.
• Disposal Guidelines:
  • Dispose of used media according to institutional biosafety protocols.
  • Autoclave if required before disposal.

Why Choose Gibco™ HBSS (With Calcium, Magnesium)?

✅ Provides essential ions for maintaining cell viability during transport & washing

✅ Sterile, pre-filtered, ready-to-use formulation ensures reproducible results

✅ Phenol red-free for reduced background in imaging & fluorescence assays

✅ Manufactured under cGMP and ISO 13485 quality standards

✅ Long shelf life (36 months) and stable storage at room temperature

Gibco™ HBSS (With Calcium, Magnesium) is an essential solution for cell culture workflows, tissue processing, and reagent preparation, ensuring high reproducibility and minimal interference in molecular biology and biopharmaceutical applications.