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Specifications:
| Application | molecular biology | ||
| Storage Temperature | Ambient | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Bio-Rad | ||
| Product Grade | Molecular Biology | ||
Bio-Rad 10% Tween 20 is a nonionic surfactant detergent widely used in molecular biology, immunoassays, and protein research. This 5 mL reagent is included in the ELISA Kit Reagent Refill Package (#166-2401EDU) and is compatible with the ELISA Immuno Explorer™ Kit (#166-2400EDU).
Tween 20 is commonly used as a blocking agent, emulsifier, and washing buffer additive, particularly in ELISA, Western blotting, and immunohistochemistry (IHC) assays. It reduces non-specific binding and enhances antibody-antigen interactions in immunoassays.
Key Features & Benefits
1. High-Purity, Laboratory-Grade Detergent
- Nonionic surfactant, ensuring gentle interactions with proteins and antibodies.
- Ideal for reducing background noise in ELISA, Western blot, and immunoassays.
- Helps solubilize proteins and lipids, improving experimental reproducibility.
2. Essential for Immunoassay Applications
- Included in the ELISA Kit Reagent Refill Package for ELISA Immuno Explorer™ Kit.
- Used as a washing buffer additive to reduce non-specific binding.
- Compatible with HRP (Horseradish Peroxidase) enzyme-based detection systems.
3. Versatile Detergent for Molecular Biology & Protein Research
- Suitable for membrane washing in blotting protocols.
- Used in buffer formulations for protein purification.
- Prevents protein aggregation in biochemical reactions.
Specifications
| Property | Details |
|---|---|
| Chemical Name | Polysorbate 20 (Tween 20) |
| Concentration | 10% (v/v) |
| Form | Liquid Detergent |
| Volume | 5 mL |
| Application | ELISA, Western blot, protein purification |
| Product Type | Nonionic surfactant detergent |
| Compatible Kits | ELISA Immuno Explorer™ Kit (#166-2400EDU) |
| Storage Conditions | Room Temperature |
| Shipping Condition | Ambient |
Applications
1. ELISA & Immunoassays
- Used as a washing buffer additive in ELISA to minimize non-specific binding.
- Compatible with HRP enzyme-linked detection for high-sensitivity assays.
- Helps maintain protein structure during immunoassays.
2. Western Blotting & Protein Analysis
- Used in blocking buffers to prevent non-specific antibody binding.
- Improves protein transfer efficiency in Western blotting.
- Minimizes background noise in blotting and immunodetection protocols.
3. Molecular Biology & Biochemical Research
- Component of membrane washing solutions.
- Solubilizes membrane proteins for extraction and purification.
- Used in cell culture media as an emulsifier.
Pack Size & Catalog Number
| Pack Size | Catalog Number (SKU) |
|---|---|
| 5 mL | Part of ELISA Kit Reagent Refill Package (#166-2401EDU) |
Storage & Handling
- Store at room temperature.
- Mix gently before use to ensure homogeneity.
- Avoid contamination by using sterile pipettes.
The Bio-Rad 10% Tween 20 (5 mL) is a high-quality, nonionic detergent designed for ELISA, Western blotting, and immunoassay applications. Included in the ELISA Kit Reagent Refill Package, it enhances protein interactions and reduces background noise in molecular biology research.