Thermo Scientific™ 96 Well Black/Clear Bottom Plate, TC Surface

The Thermo Scientific™ Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base (Catalog No. 165305) are specially designed for advanced fluorescence and luminescence applications in cell culture and microscopy. With a combination of black polystyrene walls and clear optical bottoms, these plates enhance signal detection and minimize background interference, making them ideal for high-throughput screening (HTS) and other quantitative assays.

Key Features:

1. Design and Material:
   • Black polystyrene walls: Minimize back-scattered light and background fluorescence, enhancing fluorescence assay performance.
   • Clear optical bottom: Ensures high optical clarity for microscopic applications and fluorescence/luminescence assays.
   • Solid structure: Combines a durable polymer base with virgin crystalline polystyrene for consistent performance in HTS applications.
2. Enhanced Assay Performance:
   • Fluorescence Measurements: Optimized for fluorescence with reduced autofluorescence and crosstalk.
   • Luminescence Measurements: Available in white variants for maximum signal reflection and minimal autoluminescence.
   • Volume Capacity: 50–200 μL working range per well ensures compatibility with a wide range of protocols.
3. Surface Treatment:
   • Tissue Culture (TC) Surface: Enhances cell attachment, growth, and viability, making these plates ideal for cell-based assays.
4. Convenience and Sterility:
   • Pre-Sterilized and Lidded: Sterile plates with fitted lids to maintain sample integrity and prevent contamination.
   • ANSI Standards Compliance: Designed to meet ANSI/SBS 1-2004 standards for compatibility with robotic and automated systems.
5. Customizable Features:
   • Available with customized barcoding for inventory management in HTS laboratories.
   • Suitable for fluorescence-based HTS, microscopic imaging, and luminescence assays.
6. Environmentally Controlled Manufacturing:
   • Produced in a clean environment to ensure product quality and sterility.

Applications:

1. Fluorescence Assays:
   • Use in fluorescence measurements to study cellular processes, receptor-ligand interactions, and protein-protein binding.
2. Microscopy Applications:
   • Ideal for high-resolution imaging of cell monolayers and tissue culture experiments.
3. Luminescence Assays:
   • Supports luminescent-based detection for enzymatic activity, ATP quantification, and reporter assays.
4. High-Throughput Screening (HTS):
   • Compatible with automated liquid handlers for large-scale experiments and drug discovery workflows.

Technical Specifications:

Use Cases:

1. Drug Discovery:
   • Ideal for high-throughput drug screening due to its compatibility with automated systems and fluorescence-based readouts.
2. Cell-Based Assays:
   • Supports cell adhesion and growth for toxicity assays, cell proliferation studies, and receptor-ligand interaction analysis.
3. Microscopy Imaging:
   • Optical clarity supports detailed cell and tissue imaging in fluorescence microscopy applications.
4. Biomolecular Studies:
   • Ideal for enzymatic reactions, protein-binding studies, and nucleic acid detection using fluorescence and luminescence methods.
5. Clinical and Pharmaceutical Research:
   • Used in diagnostic applications and therapeutic research requiring precise fluorescence/luminescence quantification.

Packaging Information:

• Pack Size: 10 plates per pack.
• Case Size: 30 plates per case.
• Sterilization: Sterile for immediate use in sensitive applications.

For researchers requiring precision and reliability in fluorescence and luminescence-based cell culture and screening applications, the Thermo Scientific™ 96-Well Black/Clear Bottom Plate, TC Surface delivers consistent, high-performance results in a wide range of laboratory workflows.