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Specifications:
| Application | molecular biology | ||
| Storage Temperature | –80°C | ||
| Product Type | Antibiotic | Forms | lyophilized powder |
| Product Brand | Selleckchem | ||
| Product Grade | Molecular Biology | Formula | C₁₀H₁₃N₅O₄ |
Zidovudine (AZT, Azidothymidine, NSC 602670)
Zidovudine (ZDV) is a nucleoside analogue reverse transcriptase inhibitor widely used in the treatment of HIV. It also plays a significant role in genome editing research, particularly in CRISPR-mediated knock-in and knockout studies.
Key Features
- Mechanism of Action:
- Inhibits reverse transcriptase by mimicking nucleosides, blocking viral DNA synthesis.
- Decreases homologous directed repair (HDR) efficiency and enhances knockout efficiency in CRISPR-mediated gene editing.
- High Purity:
- ≥100% purity ensures consistency in experimental outcomes.
- DNase-, RNase-, and protease-free for molecular biology applications.
- Versatile Applications:
- Effective in nucleic acid and protein research.
- Applicable in mitochondrial DNA studies and signal transduction analysis.
Chemical and Physical Properties
| Property | Details |
|---|---|
| Synonyms | Azidothymidine, NSC 602670 |
| Molecular Formula | C₁₀H₁₃N₅O₄ |
| Molecular Weight | 267.24 g/mol |
| CAS Number | 30516-87-1 |
| Melting Point | — |
| Form | Powder, 10 mM in DMSO |
| Stability | Stable for 3 years at -20°C (powder) |
| Storage Conditions | -20°C for powder, -80°C in solvent |
| Solubility | - DMSO: 53 mg/mL (198.32 mM) |
| - Water: 53 mg/mL | |
| - Ethanol: 18 mg/mL |
Applications
- Antiviral Research:
- Potent inhibitor of HIV-1 reverse transcriptase.
- Used in studies for mother-to-child transmission of HIV.
- Genome Editing (CRISPR):
- Decreases sequence-specific knock-in events.
- Enhances knockout efficiency in CRISPR workflows.
- Cell-Based Studies:
- Evaluates antiviral activity in human C8166 cells and PBMCs.
- Assesses effects on mitochondrial DNA and signal transduction pathways.
- Molecular Biology:
- RNA and DNA extraction and purification workflows.
- Inhibits protein kinase C (PKC) and modulates receptor-mediated signaling.
Experimental Data
| Cell Line | Concentration | Incubation Time | Activity |
|---|---|---|---|
| Human C8166 cells | — | — | Inhibits HIV-1-induced cytopathic effects; EC₅₀ = 4e-06 μM |
| PBMCs | 1 μM | — | Inhibits HIV-1 TEKI replication; IC₅₀ = 0.00014 μM |
| Human H9 cells | — | 6 days | Reduces HIV-1 p24 antigen expression; IC₅₀ = 0.3 nM |
Preparation and Handling
- Stock Solutions:
- Prepare in DMSO, water, or ethanol.
- Aliquot and store at -20°C to avoid repeated freeze-thaw cycles.
- In Vivo Formulation:
- Combine with solvents like PEG300, Tween 80, and ddH₂O for use in animal studies.
Safety Information
- Toxicity: Classified as a hazardous substance.
- Precautions:
- Handle with protective equipment (gloves, goggles, lab coat).
- Use in well-ventilated areas or under a fume hood.
Related Products
| Related Targets | Related Products | Related Compound Libraries |
|---|---|---|
| HBV RT, HIV RT, RT | Dapivirine (TMC120), Salicylanilide | - Antiviral Compound Library |
| Fangchinoline | - Anti-infection Compound Library | |
| - Gut Microbial Metabolite Library |
References
- Chiang G, et al., Clin Ther, 1996.
- Panther LA, et al., J Med Virol, 1999.
- Wang H, et al., Biochim Biophys Acta, 1996.
For more references, visit https://clinicaltrials.gov.
Zidovudine is a trusted choice for antiviral and genome editing research, offering high purity and proven effectiveness in nucleic acid-related workflows.
- Pack Size: 1g 25mg 10 mM x 1 mL in DMSO
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