Thermo Scientific™ Nitrocellulose Membranes, 0.45 μm

Thermo Scientific™ 0.45 µm Nitrocellulose Membranes are designed for superior protein binding performance in Western blotting, dot-blot assays, and other protein or nucleic acid detection methods. Made from 100% pure nitrocellulose, these membranes exhibit strong protein affinity, low background signal, and broad compatibility with chemiluminescence, fluorescence, and radiographic detection techniques.

The 0.45 µm pore size is ideal for transferring proteins >20 kDa and nucleic acids >300 bp, and provides the optimal balance between retention and flow for common blotting procedures. With a no-alcohol pre-wetting requirement, these membranes simplify workflow and ensure consistent performance.

Key Features and Benefits

• High binding affinity for proteins—ideal for Western blotting
• Compatible with multiple detection platforms: chemiluminescence, fluorescence, chromogenic, radiolabeling
• No alcohol pre-wet required—membranes are ready to use
• Low background signal—produces clean, publication-quality blots
• Multiple formats available—choose between rolls or pre-cut sheets
• Stable and durable—resistant to common detection reagents and can be steam-sterilized

Available Formats and Ordering Information

*Based on standard mini-gel size (8 × 10 cm)

Applications

• Western blotting (chemiluminescent and fluorescent detection)
• Dot blot assays
• Protein and nucleic acid transfer
• Immunodetection workflows
• Routine or high-sensitivity analytical blotting

Storage Guidelines

• Store flat at ambient temperature
• Avoid chemical vapors and sunlight exposure
• Highly flammable—keep away from flame and heat sources
• Autoclavable at 121°C for sterilization

Thermo Scientific™ Nitrocellulose Membranes (0.45 µm) offer a dependable solution for high-performance Western blotting and protein detection. With excellent protein binding capacity, low background, and broad method compatibility, these membranes are a gold standard in protein transfer workflows.