Thermo Scientific™ Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kits

The Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit provides an exceptionally accurate and sensitive method for quantifying low concentrations of double-stranded DNA (dsDNA). Designed specifically for fluorescence-based measurement using Qubit Fluorometers, this assay enables precise quantification of DNA samples that are too dilute for reliable measurement using traditional UV absorbance methods.

The kit features a ready-to-use 1X working solution, eliminating the need for reagent dilution and simplifying workflow. It offers outstanding selectivity for dsDNA over RNA, single-stranded DNA, proteins, and free nucleotides, ensuring accurate quantification even in the presence of contaminants commonly found in molecular biology samples.

With its ultra-sensitive detection range of 0.1–120 ng, the Qubit HS Assay Kit is ideal for next-generation sequencing (NGS), PCR, and other applications requiring accurate measurement of low-concentration DNA samples.

Key Features

Ultra-High Sensitivity for Low DNA Concentrations

• Detects dsDNA from as low as 0.1 ng
• Ideal for dilute or precious samples
• Ensures accurate quantification for critical sequencing workflows

Ready-to-Use 1X Assay Format

• Pre-formulated working solution eliminates dilution steps
• Reduces preparation time and improves workflow efficiency
• Ensures consistent and reproducible results

Exceptional Selectivity for Double-Stranded DNA

• Highly selective for dsDNA over:
  • RNA
  • Single-stranded DNA
  • Proteins
  • Free nucleotides
• Minimizes interference from contaminants

Fast and Simple Workflow

• Requires only 1–20 μL sample volume
• Results available in minutes
• Minimal hands-on time

Superior Accuracy Compared to UV Absorbance Methods

• Fluorescence-based detection provides precise DNA quantification
• Ideal for sensitive downstream applications such as NGS library preparation

Applications

The Qubit 1X dsDNA HS Assay Kit is ideal for applications requiring precise quantification of low DNA concentrations, including:

• Next-generation sequencing (NGS) library preparation
• DNA quantification before Illumina sequencing
• PCR and qPCR workflows
• DNA extraction and purification workflows
• Genomic DNA quantification
• Molecular cloning applications
• Research in genomics, biotechnology, and molecular biology

Instrument Compatibility

Compatible with the following Invitrogen Qubit Fluorometers:

• Qubit™ 2 Fluorometer
• Qubit™ 3 Fluorometer
• Qubit™ 4 Fluorometer
• Qubit™ Flex Fluorometer

Kit Contents and Shipping

Technical Specifications

Advantages for Genomics and Molecular Biology Laboratories

• Ultra-sensitive DNA quantification for low-concentration samples
• Essential for accurate Illumina NGS library preparation workflows
• Highly selective and reliable fluorescence-based detection
• Fast and easy workflow with minimal preparation
• Compatible with all Qubit Fluorometer models
• Ideal for sequencing, PCR, and molecular biology applications

The Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit is a powerful and reliable solution for precise quantification of low-concentration double-stranded DNA. Its high sensitivity, exceptional selectivity, and ready-to-use format make it an essential tool for genomics laboratories, sequencing facilities, and molecular biology researchers requiring accurate DNA measurement for critical applications such as next-generation sequencing, PCR, and cloning workflows.