Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

The Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X) is a ready-to-use, highly effective solution designed to prevent proteolytic degradation of proteins during cell lysis and protein extraction. This EDTA-free formulation ensures compatibility with specialized downstream applications, such as isoelectric focusing.

Key Features:

1. Ready-to-Use Format:
   • 100X concentrated stock solution for easy preparation.
   • Requires only 10 μL per 1 mL of lysis buffer for complete protease protection.
2. EDTA-Free:
   • Ensures compatibility with metalloprotease-sensitive processes, including 2D electrophoresis.
3. Broad-Spectrum Inhibition:
   • Inhibits a wide range of proteases, including serine-proteases, cysteine-proteases, aspartic acid-proteases, and aminopeptidases.
   • Includes optimized concentrations of:
     • AEBSF
     • Aprotinin
     • Bestatin
     • E-64
     • Leupeptin
     • Pepstatin A
4. Multiple Package Sizes:
   • Available in various formats for flexibility:
     • 1 mL, 5 mL, 10 mL, and 24 x 100 μL (single-use).
5. Stable and Convenient:
   • Liquid formulation stored at 4°C, eliminating the need for reconstitution.
6. Compatible with Diverse Lysis Methods:
   • Works with Thermo Scientific Pierce Cell Lysis Buffers, sonication, French press, blender homogenization, or repetitive freeze/thaw cycling.

Applications:

1. Protein Protection:
   • Prevents degradation of intact, active cellular proteins during lysis and extraction.
2. Protease Activity Screening:
   • Inhibits proteases in lysates to maintain protein integrity.
3. Cellular Process Studies:
   • Enables the study of proteolytic regulation in cellular mechanisms.
4. Lysis Buffer Supplement:
   • Compatible with most commercial and homemade detergent-based lysis buffers.

Specifications:

Advantages:

1. High Efficiency:
   • Effectively inhibits a broad range of proteases, reducing protein degradation.
2. Convenience:
   • Easy-to-use liquid format minimizes preparation time and reduces handling errors.
3. Versatile:
   • Works with a variety of extraction methods and buffer systems.
4. EDTA-Free Option:
   • Ensures compatibility with metalloprotease-sensitive processes and electrophoretic techniques like 2D electrophoresis.

Storage and Handling:

• Store at 4°C in its original container.
• Avoid repeated freeze/thaw cycles to maintain inhibitor activity.

Product Details:

Related Products:

• Halt™ Protease Inhibitor Single-Use Cocktail (100X)
• Thermo Scientific Pierce Cell Lysis Buffers

The Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X) is an essential reagent for preventing proteolytic degradation in protein samples, ensuring the integrity and activity of cellular proteins during lysis and extraction. Its broad-spectrum inhibition and ready-to-use format make it an indispensable tool for protein research and cellular studies.