Thermo Scientific™ Zeba™ Spin Desalting Plates, 40K MWCO

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Sample volume: 20 to 100 μL

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Specifications:
Application Size Exclusion Chromatography
Storage Temperature 2-8°C
Product Type columns
Product Brand Thermo Fisher Scientific™
Product Grade Molecular Biology

The Thermo Scientific™ Zeba™ Spin Desalting Plates (40K MWCO) are high-throughput, centrifuge-compatible tools for rapid desalting and buffer exchange of protein samples. Designed specifically for macromolecules larger than 40 kDa, these 96-well filter plates remove small contaminants (<2 kDa) such as salts, dyes, excess labels, and crosslinkers from small-volume samples ranging from 20 to 100 µL.

Each well is pre-packed with a proprietary, high-efficiency size-exclusion resin that offers superior protein recovery while withstanding centrifugal force without bead deformation. These plates are ready to use—no hydration or resin packing required—making them ideal for processing multiple protein samples simultaneously in proteomics, antibody purification, enzyme assays, and biochemical research workflows.

Key Features & Benefits

  • High Molecular Weight Cut-Off (40K MWCO)
    Effectively separates proteins and macromolecules (>40 kDa) from low molecular weight contaminants (<2 kDa).
  • High Recovery, Even at Low Concentrations
    Optimized resin formulation minimizes protein loss and maximizes desalting efficiency.
  • Fast and Efficient Workflow
    Complete desalting in 5 minutes without the need for dilution, fraction collection, or complex equipment.
  • Flexible Throughput
    Available in 2- and 4-plate packs; ideal for labs requiring reliable, repeatable results with small sample volumes.
  • Pre-packed and Ready-to-Use
    Eliminates the time and variability associated with manual plate preparation.
  • Compatible with Standard Centrifuges
    Designed for use in common laboratory plate rotors.

Applications

  • Buffer exchange prior to mass spectrometry, ELISA, HPLC, or electrophoresis
  • Removal of detergents, dyes, salts, and excess chemical reagents after labeling or conjugation
  • Desalting of antibodies, enzymes, recombinant proteins, or protein complexes
  • Sample cleanup before protein interaction studies or functional assays
  • High-throughput preparation of protein samples in drug discovery and biotechnology research

Technical Specifications

FeatureDetails
Product TypeSpin Desalting Plate
Format96-well Filter Plate
Molecular Weight Cut-Off40,000 Da
Resin TypeProprietary size-exclusion resin
Sample Volume Range20 to 100 µL per well
UseSingle-use disposable
Centrifuge CompatibilityYes (plate rotor)
Contaminant Removal>95% of salts and low MW molecules
Protein Target Range>40 kDa
Storage Conditions4°C to 8°C
Product LineZeba™
Catalog NumberQuantitySample Volume Range
A577672 plates20 to 100 µL
A577684 plates20 to 100 µL

The Zeba™ Spin Desalting Plates, 40K MWCO provide a simple yet highly effective solution for small-volume, high-throughput protein desalting and buffer exchange. Whether used for sample preparation in proteomics, enzyme workflows, or antibody studies, these 96-well plates deliver reliable, reproducible results with minimal protein loss. Their ease of use and robust performance make them an ideal choice for labs focused on efficiency, precision, and throughput in protein purification and analysis.

  • Pack Size:   2/pk 4/pk

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