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Thermo Scientific™ Tween 20 Washing Buffer, 1.0% in PBS (20X)
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High-Strength PBS-T Wash and Blocking Buffer for ELISA, Western Blotting, and Immunoassays
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Ambient | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Tween 20 Washing Buffer, 1.0% in PBS (20X) is a concentrated phosphate-buffered saline detergent solution formulated for efficient washing and blocking in antibody-based detection workflows. Commonly referred to as PBS-T, this buffer contains 1.0% Tween 20, a gentle nonionic surfactant that enhances washing performance and reduces nonspecific binding in immunoassays.
Supplied as a convenient 20X stock concentrate, this solution supports rapid preparation of working-strength wash buffer for routine use in ELISA, Western blotting, and other plate- or membrane-based immunodetection assays.
Key Features and Benefits
Enhanced Washing Efficiency with 1.0% Tween 20
The increased detergent concentration improves removal of unbound antibodies, proteins, and assay reagents, helping to reduce background signals.
Ideal for Immunoassay Workflows
Designed for consistent performance in:
- ELISA plate washing
- Western blot membrane washing
- General immunoassay protocols
Suitable for Blocking Applications
Also used as a blocking buffer in plate-based assays to minimize nonspecific adsorption and improve assay sensitivity.
Convenient 20X Concentrated Format
Delivered as a ready-to-dilute stock solution, reducing preparation time and ensuring reproducible buffer composition.
Stable and Easy to Store
Recommended for storage at ambient temperatures, simplifying laboratory handling and logistics.
Applications
Thermo Scientific™ Tween 20 Washing Buffer is commonly used for:
- Wash steps in ELISA protocols
- Western blot membrane washing
- Blocking buffer preparation for plate-based immunoassays
- Removal of excess immunoreagents between incubation steps
- Reduction of nonspecific antibody binding
Product Specifications
| Parameter | Description |
|---|---|
| Product Name | Tween 20 Washing Buffer |
| Composition | 1.0% Tween 20 in PBS |
| Concentration | 20X Stock Solution |
| Physical Form | Liquid |
| Chemical Type | Buffer |
| Recommended Storage | Ambient temperatures |
| Primary Use | ELISA, Western blot, immunoassays, blocking |
Ordering Information
| Catalog Number | Pack Size | Alternate Name |
|---|---|---|
| J62844.K2 | 1 L | J62844-K2 |
| J62844.K3 | 2 L | J62844-K3 |
Thermo Scientific™ Tween 20 Washing Buffer, 1.0% in PBS (20X), is a reliable high-strength PBS-T solution for laboratories performing ELISA, Western blotting, and other immunoassays. Its optimized detergent formulation ensures efficient washing, reduced nonspecific binding, and improved assay clarity. Supplied in a convenient concentrated format, it is an essential reagent for reproducible immunodetection workflows.
- Pack Size: 1L 2L