Thermo Scientific™ Resazurin sodium salt, pure, high purity biological stain

Thermo Scientific™ Resazurin Sodium Salt is a high-purity, versatile biological stain widely used as a redox indicator and viability assay reagent in microbiology, cell biology, and biochemistry. It is particularly valued for its reliability, sensitivity, and safety in applications that monitor cellular respiration, metabolic activity, or oxygen levels.

Resazurin sodium salt is a non-toxic, water-soluble dye that undergoes a colorimetric and fluorometric change in response to redox reactions, transitioning from blue (oxidized state) to pink (reduced state) or fluorescing strongly in reduced forms. This makes it ideal for viability assays and monitoring metabolic activity in live cells and microbial cultures.

Key Features

1. High Purity: Manufactured to high-purity standards, ensuring reproducibility and accuracy in sensitive biological applications.
2. Dual Detection Capability: Offers both colorimetric and fluorometric detection, providing flexibility for diverse experimental setups.
3. Non-Toxic and Water-Soluble: Safe for live-cell assays, minimizing interference with cellular function.
4. Versatile Applications: Suitable for use in microbial viability tests, metabolic activity monitoring, oxygen detection, and biocompatibility assays.
5. Reliable Sensitivity: Detects even small changes in metabolic activity, making it ideal for low-level respiration studies.

Specifications

Chemical Properties

• Chemical Formula: C12H6NNaO4
• Molecular Weight: 251.17 g/mol
• Appearance: Blue crystalline powder
• Color Change: Blue (oxidized) → Pink (reduced)
• Excitation/Emission Wavelengths (Fluorometric Detection):
  • Excitation: ~560 nm
  • Emission: ~590 nm

Applications

1. Microbial Viability and Metabolic Activity Assays

• Used to measure the metabolic activity of bacteria, yeast, and other microorganisms in culture.
• Monitors cellular respiration through the reduction of resazurin to resorufin (pink, fluorescent).

2. Cell Viability and Cytotoxicity Testing

• Incorporated into assays to evaluate the viability of mammalian cells and other eukaryotic systems.
• Commonly used in drug screening and cytotoxicity studies to monitor metabolic health.

3. Oxygen and Redox Monitoring

• Applied as a redox indicator in experiments studying oxygen consumption or oxidative stress in cells.
• Useful in assessing anaerobic conditions in microbiological cultures.

4. Environmental and Industrial Applications

• Monitors microbial activity in environmental samples, such as soil or wastewater.
• Utilized in fermentation processes to track metabolic changes in bioreactors.

Preparation and Usage

1. Solution Preparation:
   • Dissolve the required quantity of resazurin sodium salt in sterile distilled water to prepare a stock solution (e.g., 0.01% w/v).
   • Store the solution at 2–8°C in the dark to maintain stability.
2. Assay Integration:
   • Add an appropriate volume of the resazurin solution to the culture medium or test sample.
   • Incubate at the desired temperature for the recommended time, typically 1–4 hours for microbial or cell-based assays.
3. Detection:
   • Colorimetric: Observe the color change from blue (oxidized) to pink (reduced).
   • Fluorometric: Measure fluorescence using a plate reader or fluorometer (Excitation: ~560 nm, Emission: ~590 nm).

Storage and Stability

• Storage Conditions: Store at room temperature in a dry, dark environment.
• Stability: Protect from light and moisture to ensure long shelf life and activity.

Benefits

1. Sensitive and Reliable: Detects small changes in metabolic activity, ensuring precise results in viability assays.
2. Non-Toxic: Safe for use in live-cell assays, preserving cell health and function during experiments.
3. Multi-Functional: Compatible with a variety of biological systems, from microbes to mammalian cells.
4. Convenient Preparation: Easily soluble in water, facilitating rapid assay setup.

Precautions

• Use appropriate personal protective equipment (PPE) when handling the powder.
• Handle under low-light conditions to prevent degradation.
• Dispose of waste in accordance with local regulations.

The Thermo Scientific™ Resazurin Sodium Salt, Pure, High-Purity Biological Stain is an indispensable tool for laboratories conducting cell viability assays, microbial activity studies, or oxygen/redox monitoring. Its versatility, sensitivity, and non-toxic nature make it ideal for a wide range of applications in clinical, research, and industrial microbiology.