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Thermo Scientific™ Pierce™ Trifluoroacetic Acid (TFA), Sequencing grade, 99.5%
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CAS Number :
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Specifications:
| Application | Next Generation Sequencing, molecular biology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | Formula | C₂HF₃O₂ |
Pierce™ Sequencing Grade TFA is a high-purity trifluoroacetic acid specially manufactured for use as an ion-pairing agent in reverse-phase high-performance liquid chromatography (RP-HPLC) of peptides and proteins. Its ultra-low UV absorbance, volatile nature, and formulation quality make it ideal for applications where reproducibility, sensitivity, and instrument compatibility are critical.
This sequencing-grade TFA is rigorously purified and packaged under nitrogen in amber glass bottles with PTFE-lined fluorocarbon caps, ensuring chemical stability, safety, and minimal degradation upon storage.
Key Features & Benefits
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≥99.5% Purity
High-grade reagent suitable for LC-MS, peptide mapping, HPLC, and sequencing workflows. -
Exceptional UV Transparency
Enables low-background detection at short UV wavelengths, ideal for sensitive peptide quantification. -
Stable, Inert Packaging
Supplied in amber glass to prevent degradation; sealed under nitrogen to prevent moisture absorption. -
Volatile and MS-Compatible
Easily removed by evaporation; ideal for downstream mass spectrometry applications. -
Flexible Formats
Also available in 1 mL ampoules and 1 g, 100 g, and 10 × 1 g options to suit small- and large-scale workflows.
Recommended Applications
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Reverse-Phase HPLC (RP-HPLC) Peptide/Protein Separation
Functions as an ion-pairing agent to enhance peak sharpness and retention. -
Peptide Sequencing & Proteomics
Supports high-resolution mapping and quantification of tryptic peptides and protein fragments. -
Solid-Phase Peptide Synthesis (SPPS)
Used for cleavage and side-chain deprotection of synthetic peptides. -
Amino Acid Analysis
TFA acts as a solubilizer and modifier in chromatographic amino acid profiling. -
Mass Spectrometry Sample Prep
Volatile nature makes it ideal for MS workflows requiring minimal background interference.
TFA Concentration: Weight/Volume vs. Volume/Volume
| Specification | Definition | Implication |
|---|---|---|
| 0.1% w/v | 1 gram of TFA per liter of solution | More acidic than 0.1% v/v |
| 0.1% v/v | 1 mL of TFA per liter of solution | Less acidic; 1.53 g/L due to density |
| Note: | Density of TFA = 1.53 g/mL | Clarify w/v or v/v for reproducibility |
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Trifluoroacetic Acid (TFA), Sequencing Grade |
| Purity | ≥99.5% (TFA) |
| CAS Number | 76-05-1 |
| Molecular Weight | 114.02 g/mol |
| Packaging | Amber glass with PTFE-lined fluorocarbon cap |
| Storage Conditions | 20–25 °C, cool, dry, away from light |
| Hazard Classification | Corrosive, volatile – handle in fume hood |
| Catalog Number | Quantity / Format | Packaging Description | Use Case |
|---|---|---|---|
| 28904 | 10 × 1 mL | Sealed glass ampoules (pre-measured volume) | Ideal for precise volume-based (v/v) HPLC/mobile phase prep (e.g., 0.1% TFA) |
| 28902 | 10 × 1 g | Sealed glass ampoules (pre-weighed mass) | Ideal for accurate weight-based (w/v) solution prep (e.g., 0.1% TFA w/v) |
| 28903 | 100 g | Amber bottle | Suitable for frequent TFA use and larger-scale lab workflows |
| 28901 | 500 mL | Amber glass bottle with PTFE-lined cap | Best for high-throughput workflows or shared lab reagent stocks |
Thermo Scientific™ Pierce™ Sequencing Grade TFA is an ultra-pure ion-pairing agent optimized for reverse-phase HPLC, MS sample prep, and peptide sequencing. Its consistent lot-to-lot purity, inert packaging, and compatibility with MS detection make it the gold standard for protein and peptide workflows requiring high sensitivity and reproducibility.
- Pack Size: 100g 500 mL 10 x 1 mL 10 x 1 g