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Thermo Scientific™ Oxoid™ Violet Red Bile Glucose (VRBG) Agar (ISO)
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for examination of food, animal feed, and environmental samples, for the presence of indicator groups such as coliforms
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated) is a selective medium specifically formulated for the detection and enumeration of Enterobacteriaceae in food, animal feed, and environmental samples. The medium adheres to ISO 21528 and ISO 11133:2014 standards, providing reliable results for food hygiene and safety monitoring. By targeting Enterobacteriaceae rather than just coliforms, this medium enhances the assessment of food processing and contamination.
Key Features
- Selective for Enterobacteriaceae:
- Contains neutral red, crystal violet, and bile salts to inhibit Gram-positive bacteria while promoting Enterobacteriaceae growth.
- Glucose as Carbon Source:
- Modified formulation includes glucose for improved recovery of all Enterobacteriaceae, allowing accurate enumeration.
- Easy-to-Interpret Results:
- Enterobacteriaceae ferment glucose, reducing pH and forming purple-pink colonies surrounded by halos of precipitated bile salts.
- Standards Compliant:
- Tested for performance according to ISO 21528 and ISO 11133:2014 for reliable and reproducible results.
- Broad Applications:
- Suitable for food, animal feed, and environmental testing to monitor hygiene, safety, and spoilage potential.
Specifications
| Property | Details |
|---|---|
| Description | Violet Red Bile Glucose (VRBG) Agar (ISO) |
| Form | Powder |
| Product Type | Agar |
| Quantity | 500 g |
| Shelf Life | 5 Years |
| Storage Conditions | 10°C to 30°C |
| Catalog Numbers | CM1082B (500g), CM1082R (2.5kg) |
Applications
- Food Microbiology:
- Detect and enumerate Enterobacteriaceae in raw and processed food products.
- Animal Feed Testing:
- Evaluate microbial contamination in animal feed samples.
- Environmental Monitoring:
- Monitor hygiene and microbial contamination in food production and handling environments.
- Process Validation:
- Assess heat treatment and identify process failures, under-processing, or post-processing contamination in food manufacturing.
Preparation Instructions
- Suspend 41.5g of VRBG Agar powder in 1 liter of distilled water.
- Heat gently to dissolve completely.
- Bring to a boil while stirring to avoid localized overheating.
- Do not autoclave. Cool to 45-50°C, pour into sterile Petri dishes, and allow to solidify.
Advantages
- Highly Selective: Effectively suppresses Gram-positive organisms, ensuring accurate results for Enterobacteriaceae.
- Reliable Indicators: Neutral red and glucose fermentation produce distinct purple-pink colonies for easy interpretation.
- Standards-Compliant: Validated according to ISO standards for performance and consistency.
- Versatile Applications: Suitable for food safety, environmental monitoring, and process validation.
Packaging
- Quantity: Available in 500g (CM1082B) and 2.5kg (CM1082R).
- Yield: Prepares 12.1 liters per 500g.
Why Choose VRBG Agar?
- Recognized for its reliability in food safety and quality control testing.
- Complies with international standards for microbiological analysis.
- Backed by Thermo Scientific’s expertise and commitment to quality.
- Pack Size: 500g 2.5kg