Thermo Scientific™ Oxoid™ ONE Broth-Listeria

Oxoid™ ONE Broth-Listeria is an innovative selective enrichment broth specifically designed for the detection and recovery of Listeria species, including Listeria monocytogenes, from food samples within 24 hours. The broth's optimized formulation ensures effective resuscitation and growth of even low-level Listeria spp., making it a powerful tool for food safety testing in research, clinical, and industrial environments.

Key Features

1. Rapid Enrichment: Allows selective enrichment of Listeria species in food samples within 24 hours, ensuring timely results.
2. Optimized Composition:
   • Balances peptones, carbohydrates, and salts for efficient recovery.
   • Formulated to promote the resuscitation of stressed or low numbers of Listeria spp.
3. Supplement Requirement: Works in conjunction with ONE Broth-Listeria Supplement (Part No. SR0234E), which enhances the selectivity of the medium.
4. Visual Characteristics:
   • Dehydrated Medium: Straw-colored, free-flowing powder.
   • Selective Supplement: Orange/yellow freeze-dried pellet.
   • Prepared Medium: Dark straw-colored, clear liquid.

Typical Formulation

Formulation Directions

1. Suspend 22g of ONE Broth-Listeria Base in 500mL (44g/L) of distilled water.
2. Mix well and sterilize by autoclaving at 121°C for 15 minutes.
3. Cool the medium to below 50°C.
4. Aseptically add the contents of ONE Broth-Listeria Selective Supplement (SR0234E) reconstituted as directed.

Testing Protocol

1. Sample Preparation:
   • Add 25g of food sample to 225mL of prepared ONE Broth-Listeria.
   • Stomach (homogenize) the sample for at least 30 seconds.
2. Incubation:
   • Incubate the broth at 30°C for 24 hours without agitation.
3. Plating:
   • After incubation, gently agitate the sample bag.
   • Using a microbiological loop, inoculate 10μL onto a suitable agar medium, such as:
     • Brilliance Listeria Agar (CM1080) with SR0222 and SR0228 supplements.
   • Incubate plates at 37°C for 24-48 hours.
4. Confirmation:
   • Identify presumptive colonies as Listeria monocytogenes or Listeria spp. using methods such as:
     • Gram-staining.
     • Catalase or oxidase tests.
     • Oxoid Listeria Latex Test Kit (DR1126).
     • Additional confirmation kits like:
       • Oxoid O.B.I.S. mono (ID0600).
       • Microbact Listeria 12L (MB1128).

Applications

1. Food Safety Testing:
   • Detect and enumerate Listeria spp. in processed foods, raw food materials, and ready-to-eat products.
2. Research:
   • Study Listeria spp. recovery, resuscitation, and growth dynamics in laboratory settings.
3. Quality Assurance:
   • Ensure compliance with food safety regulations by verifying the absence of Listeria monocytogenes in production environments.

Advantages

• Efficiency: Delivers reliable results in 24 hours, streamlining testing workflows.
• Versatility: Suitable for various food sample types and industry applications.
• Enhanced Sensitivity: Effective in recovering low levels of stressed or injured Listeria spp.

Specifications

For reliable and rapid detection of Listeria species, Oxoid ONE Broth-Listeria is an essential component in microbiological food safety testing workflows.