Thermo Scientific™ Oxoid™ Buffered Listeria Enrichment Broth

The Thermo Scientific™ Oxoid™ Buffered Listeria Enrichment Broth is a highly effective medium for the selective enrichment of Listeria species in food and environmental samples. Based on the original formulation by Lovett et al., this broth has been modified to enhance its buffering capacity with the addition of potassium dihydrogen orthophosphate and disodium hydrogen orthophosphate, making it ideal for samples with acidic or challenging matrices such as fermented products.

Key Features

1. Enhanced Buffering Capacity:
   • Optimized to neutralize acidic conditions in complex food and environmental samples.
2. Selective Enrichment:
   • Formulated to promote the growth of Listeria spp. while suppressing competing organisms.
3. Broad Application:
   • Suitable for use with fermented products and environmental specimens, offering flexibility across various sample types.
4. Supplement Compatibility:
   • Compatible with a range of Listeria Selective Supplements:
     • SR0140E or SR0143E
     • SR0206E or SR0206B
     • SR0141E
5. Regulatory Compliance:
   • Conforms to food safety testing standards, supporting accurate detection and recovery of Listeria spp.

Applications

1. Food Microbiology

• Enrichment of Listeria spp. from processed and raw food samples, including:
  • Dairy products
  • Fermented foods
  • Meat and poultry

2. Environmental Microbiology

• Detection and recovery of Listeria from environmental swabs, equipment surfaces, and food production environments.

3. Regulatory and Compliance Testing

• Use in food safety workflows to ensure compliance with industry standards.

Specifications

Preparation Instructions

1. Suspend: Suspend 55g of Buffered Listeria Enrichment Broth in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation until fully dissolved.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Supplement: Aseptically add the appropriate Listeria Selective Supplement (e.g., SR0140E, SR0141E, or SR0206E).
5. Enrich: Inoculate the medium with the sample and incubate under the recommended conditions for selective enrichment of Listeria spp.

Advantages

1. Enhanced Recovery: Improved buffering capacity supports growth in acidic and complex sample matrices.
2. Selective and Effective: Suppresses non-target organisms while promoting Listeria spp. growth.
3. Flexible Use: Suitable for both food and environmental testing workflows.
4. Supplement Integration: Compatibility with various selective supplements enhances medium functionality.
5. Regulatory Support: Conforms to industry and regulatory standards for food safety testing.

Supplement Compatibility

Safety Considerations

• Cycloheximide in Supplements: Toxic if swallowed, inhaled, or in contact with skin. Wear gloves and eye/face protection during handling.
• Acriflavine: Light-sensitive; store appropriately to avoid inhibitory compound formation.

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to the product label for expiration date.

Packaging

• Product: Buffered Listeria Enrichment Broth
• Quantity: 500g or larger options available
• Yield: Approximately 9.1L per 500g

The Thermo Scientific™ Oxoid™ Buffered Listeria Enrichment Broth is an optimized medium for the selective enrichment of Listeria spp. from food and environmental samples. Its enhanced buffering capacity and compatibility with various supplements make it indispensable for microbiological testing in food safety and environmental monitoring. This broth ensures robust recovery and reliable results, supporting laboratories in meeting regulatory and quality assurance standards.