Thermo Scientific™ Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment, 500 units

Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment is a highly purified recombinant enzyme derived from E. coli, engineered to lack the 5′→3′ exonuclease activity of intact DNA Polymerase I, while retaining both 5′→3′ polymerase activity and 3′→5′ exonuclease (proofreading) activity. This enzymatic profile makes it ideal for precise DNA manipulations in molecular biology workflows where removal of the 5′→3′ exonuclease function is critical.

This Klenow fragment is extensively tested for purity and activity and is shipped with a 10X REact™ 2 Buffer for optimal performance.

Applications

• Fill-in of 5′ Overhangs
  Used to fill recessed 3′ ends or convert 5′ overhangs into blunt ends in double-stranded DNA.
• Random Primer Labeling
  Facilitates synthesis of radiolabeled or fluorescent probes via random priming techniques.
• DNA Sequencing
  Applicable for sequencing of both single-stranded and double-stranded DNA templates.
• Site-Directed Mutagenesis
  Supports generation of point mutations by incorporating specific nucleotide changes in vitro.

Key Features and Benefits

• Lacks 5′→3′ Exonuclease Activity: Prevents degradation of DNA strands during reactions.
• Retains 5′→3′ Polymerase and 3′→5′ Exonuclease Functions: Enables DNA synthesis and proofreading.
• High Purity and Performance: Validated using SDS-PAGE and functional assays.
• Ready-to-use Formulation: Supplied with REact™ 2 Buffer for optimal enzymatic conditions.
• Flexible Reaction Setup: Compatible with standard cloning and labeling workflows.

Specifications

Contents and Storage

• Supplied with:
  • 10X REact™ 2 Buffer:
    [500 mM Tris-HCl (pH 8.0), 100 mM MgCl₂, 500 mM NaCl]
  • Dilution buffer
• Storage: Store at –20 °C. Avoid repeated freeze-thaw cycles.

The Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment is a versatile and essential tool for molecular biology workflows that require DNA synthesis without strand degradation. Its balanced enzymatic profile makes it ideal for precise DNA modifications, blunt-end repair, probe generation, and mutagenesis. Backed by Thermo Fisher Scientific’s rigorous quality standards, this enzyme delivers consistent, high-performance results for research and genomic applications.