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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ FastDigest EheI is a high-quality restriction enzyme that recognizes the sequence 5′ GGC^GCC 3′ / 3′ CCG^CGG 5′, producing blunt ends. Optimized for rapid DNA digestion, FastDigest EheI achieves complete cleavage within 5–15 minutes at 37 °C using the universal FastDigest Buffer system.
As part of the FastDigest™ enzyme portfolio, EheI is fully compatible with both the FastDigest and FastDigest Green Buffers, eliminating the need for buffer changes in single, double, or multiple digestion reactions. Its optimized formulation prevents star activity and supports direct downstream workflows with DNA modifying enzymes such as T4 DNA Ligase, Klenow Fragment, T4 DNA Polymerase, and alkaline phosphatases.
Key Features
- Rapid digestion – complete cleavage in 5–15 minutes.
- Universal buffer system – 100% enzyme activity in both FastDigest and FastDigest Green Buffers.
- Direct loading – FastDigest Green Buffer includes density reagent and tracking dyes for gel electrophoresis.
- Star activity prevention – optimized buffer composition ensures high fidelity.
- Seamless workflow – downstream modifying enzymes are fully compatible with the universal buffer.
- Broad availability – part of a portfolio of 176 FastDigest enzymes.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping and SNP analysis.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | EheI |
| Recognition Sequence | 5′ GGC^GCC 3′ / 3′ CCG^CGG 5′ |
| Cleavage Product | Blunt ends |
| Isoschizomers | BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI |
| Optimal Reaction Temperature | 37 °C |
| Complete Digestion Time | 5–15 minutes |
| Compatible Buffers | 10X FastDigest Buffer, 10X FastDigest Green Buffer |
| Inactivation Temperature | 65 °C |
| Methylation Sensitivity | CpG methylation-sensitive; not Dam/Dcm-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Formats
| Catalog Number | Quantity | Reactions | Format |
|---|---|---|---|
| FD0443 | 20 μL | ~20 reactions | Enzyme + Buffers |
| FD0444 | 50 μL | ~50 reactions | Enzyme + Buffers |
Contents:
- FastDigest EheI enzyme
- 10X FastDigest Buffer (1.0 mL)
- 10X FastDigest Green Buffer (1.0 mL)
Thermo Scientific™ FastDigest EheI delivers fast, accurate, and high-fidelity restriction digestion for cloning and genotyping workflows. With blunt-end cutting precision, compatibility with the universal FastDigest Buffer system, and rapid digestion times, it streamlines molecular biology applications while eliminating workflow bottlenecks. Its reliability and flexibility make it a preferred choice for laboratories requiring efficient DNA digestion with complete downstream compatibility.
- Pack Size: 20 μL (20 Reactions) 50 μL (50 Reactions)