Thermo Scientific™ Dry-blend Buffered Packs

The Thermo Scientific™ Dry-blend Buffered Packs are convenient, pre-measured powder buffer packs designed for efficient and consistent buffer preparation in laboratory settings. These dry packs eliminate the need for weighing, pH adjustments, or stocking individual buffer components, saving time and ensuring reproducibility. They are ideal for a variety of molecular biology and biochemistry applications.

Key Features

1. Convenience:
   • Pre-measured powder packs simplify preparation—just dissolve in ultrapure water.
   • Eliminates the need for weighing, pH adjustment, and preparation of stock solutions.
2. Consistency:
   • Quality-controlled manufacturing ensures consistent buffer composition and performance.
3. Long Shelf Life:
   • Dry format buffers have an extended shelf life, reducing concerns about stability compared to liquid stock solutions.
4. Versatile Applications:
   • Wide variety of buffer types tailored for specific laboratory procedures.

Types of Buffered Packs

1. Phosphate Buffered Saline (PBS) Packs:
   • Composition: 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2.
   • Applications: Crosslinking and labeling reactions requiring amine-free conditions.
   • Makes 500 mL of buffer per pack.
2. MES Buffered Saline Packs:
   • Composition: 0.1 M MES (2-(N-morpholino) ethanesulfonic acid), 0.9% sodium chloride, pH 4.7.
   • Applications: Optimized for carbodiimide (EDC) crosslinking and conjugation reactions.
   • Makes 500 mL of buffer per pack.
3. Carbonate-Bicarbonate Buffer Packs:
   • Composition: 0.2 M carbonate-bicarbonate, pH 9.4.
   • Applications: Coating or binding antibodies and proteins onto polystyrene microplates for ELISA and other assays.
   • Makes 500 mL of buffer per pack.
4. Tris Buffered Saline (TBS) Packs:
   • Composition: 25 mM Tris, 0.15 M NaCl, pH 7.2.
   • Applications: Diluents and wash buffers for ELISA, western blotting, and general immunoassay procedures.
   • Makes 500 mL of buffer per pack.
5. Borate Buffer Packs:
   • Composition: 50 mM borate, pH 8.5.
   • Applications: Protein modification procedures requiring amine-free conditions.
   • Makes 500 mL of buffer per pack.
6. Tris-Glycine Transfer Buffer Packs:
   • Composition: 25 mM Tris, 192 mM glycine, pH 8.
   • Applications: Protein transfer from gels to blotting membranes in wet or semi-dry electrophoresis.
   • Makes 500 mL of buffer when combined with 400 mL water and 100 mL methanol.
7. Modified Dulbecco’s PBS (DPBS) Packs:
   • Composition: 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4.
   • Applications: Diluents and wash buffers for ELISA and western blotting.
   • Makes 500 mL of buffer per pack.

Applications

• ELISA and Immunoassays:
  • Used as wash buffers, diluents, or coating buffers for plate-based assays.
• Protein Electrophoresis and Transfer:
  • TBS and Tris-Glycine buffers are ideal for western blotting and gel electrophoresis workflows.
• Protein Modification:
  • Borate and MES buffers support crosslinking and conjugation reactions.
• General Lab Use:
  • PBS and DPBS are versatile for numerous molecular biology and biochemistry procedures.

Benefits

• Time-Saving:
  • Immediate use without additional preparation steps.
• Reproducibility:
  • Consistent buffer preparation ensures reliable experimental results.
• Compact Storage:
  • Dry packs require less storage space compared to pre-made liquid buffers.
• Easy Transport:
  • Lightweight and sealed foil envelopes for secure handling.

Specifications

Catalog Options

Storage and Stability

• Store in the original container in a dry, cool, and well-ventilated area.
• Protect from direct sunlight.
• Storage Temperature: 20–25°C.

For Research Use Only

These buffers are designed for laboratory research and are not for use in diagnostic procedures.

The Thermo Scientific™ Dry-blend Buffered Packs provide a convenient, consistent, and versatile solution for a wide range of laboratory applications. Their long shelf life and ready-to-use design make them an essential tool for reliable experimental workflows.