Thermo Scientific™ DreamTaq™ Green PCR Master Mix (2X)

The Thermo Scientific DreamTaq PCR Master Mix (2X) is a pre-mixed solution combining all essential components for standard PCR, including DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl₂, and dNTPs. It simplifies the PCR setup process by reducing pipetting steps, which minimizes the risk of contamination and saves time.

The master mix is available in colorless and green formats, with the green version containing tracking dyes for direct gel loading.

Key Features

1. Pre-Mixed Convenience:
   • All-in-one 2X formulation includes DreamTaq DNA Polymerase, optimized buffer, MgCl₂, and dNTPs.
   • Eliminates the need for separate component preparation.
2. Robust Performance:
   • Amplifies targets up to 6 kb from genomic DNA and 20 kb from viral DNA.
   • Retains higher sensitivity and yields compared to conventional Taq polymerase.
3. Green Format Benefits:
   • Includes tracking dyes for monitoring gel electrophoresis progress.
   • Density reagent allows for direct loading onto agarose gels without additional dye.
4. Enhanced Sensitivity:
   • Superior amplification performance with minimal optimization.
5. Wide Applications:
   • Suitable for routine PCR, genotyping, and high-throughput workflows.

Applications

• Routine PCR: Amplification of DNA fragments up to 6 kb.
• Genotyping: Reliable and high-fidelity amplification for genetic analysis.
• High-Throughput PCR: Simplified workflows for large-scale experiments.
• Direct Gel Loading: Green buffer eliminates the need for additional dyes in electrophoresis.

Product Specifications

Formats

Comparison between Thermo Scientific™ DreamTaq PCR Master Mix (2X) and Promega GoTaq Green Master Mix (2X):

Key Differences

1. Target Length:
   • DreamTaq Master Mix supports longer amplification targets (up to 20 kb for viral DNA).
   • GoTaq Green Master Mix is optimized for standard target lengths (up to 5 kb).
2. Flexibility:
   • Both offer green and colorless formats; DreamTaq's Green Buffer supports broader downstream applications like sequencing and ligation.
3. Applications:
   • DreamTaq Master Mix is better suited for amplifying longer targets or high-throughput PCR workflows.
   • GoTaq Green is focused on standard endpoint PCR workflows.

This comparison highlights DreamTaq’s ability to handle more complex workflows with longer targets, while GoTaq Green provides a solid solution for routine PCR applications. The choice between the two depends on the experimental requirements.

Advantages

1. Time-Saving and Convenient:
   • Pre-mixed solution minimizes setup time and reduces contamination risk.
2. High Sensitivity and Yield:
   • Ensures robust amplification of long DNA targets.
3. Versatility:
   • Suitable for a range of PCR applications, including routine and high-throughput PCR.
4. Direct Gel Loading (Green Format):
   • Simplifies workflows by eliminating the need for gel loading dyes.

Ordering Information

Storage and Stability

• Store at -20°C to maintain product integrity.
• Refer to the product label for the expiration date.

Intended Use

• Research Use Only. Not for use in diagnostic procedures.

The DreamTaq PCR Master Mix (2X) is an excellent choice for researchers seeking a simplified, reliable, and high-performance solution for PCR workflows.