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Thermo Scientific™ dNTP Set, 100 mM Solutions
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Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ dNTP Set is a ready-to-use, high-purity set of 100 mM aqueous nucleotide solutions consisting of dATP, dCTP, dGTP, and dTTP in separate vials. These ultra-pure deoxynucleotide triphosphates are designed for use in a broad range of molecular biology applications, including PCR, qPCR, RT-PCR, high-fidelity PCR, DNA sequencing, and cDNA synthesis.
Each nucleotide is adjusted to a pH of 7.3–7.5 with NaOH and is free of nuclease contamination and genomic DNA (human or E. coli). This set is ideal for researchers who require flexibility and control over individual nucleotide concentrations in their reaction mixes.
Key Features & Benefits
✅ High Purity & Performance
- Each dNTP is >99% pure, verified by HPLC analysis.
- Free of DNase, RNase, and human/E. coli DNA contamination.
✅ Stable & Reliable
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Demonstrates excellent stability under PCR cycling conditions and room temperature storage:
- >90% integrity after 30 PCR cycles
- >95% dNTPs remain in triphosphate form after 7 weeks at room temp
✅ Convenient Format
- Each nucleotide is provided in a separate 100 mM solution, allowing precise formulation of custom reaction mixes.
✅ Flexible Volumes
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Available in multiple pack sizes to suit low- to high-throughput labs:
- R0181 – 4 × 0.25 mL
- R0182 – 4 × 1 mL
- R0186 – 4 × 5 mL
Applications
- PCR (standard and high-fidelity)
- Quantitative real-time PCR (qPCR)
- RT-PCR and cDNA synthesis
- Long-range PCR (up to 40 kb)
- Whole genome amplification (MDA, LAMP-PCR)
- Random DNA amplification (RDA)
- DNA labeling, mutagenesis, and sequencing
Product Specifications
| Property | Details |
|---|---|
| Set Contents | 4 vials: dATP, dCTP, dGTP, dTTP |
| Concentration | 100 mM (each dNTP) |
| pH Range | 7.3–7.5 (adjusted with NaOH) |
| Purity | >99%, confirmed by HPLC |
| Form | Aqueous solution |
| Label/Dye | Unlabeled |
| Nuclease Free | Yes |
| DNA Contamination Free | Yes (human and E. coli) |
| Storage Temperature | –20°C |
| Shipping Conditions | Dry Ice |
Stability Data
- After multiple freeze-thaw cycles: No significant degradation.
- At room temperature (7 weeks): >95% remain in triphosphate form.
- Under PCR conditions (30 cycles): >90% remain intact.
Why Choose the Thermo Scientific™ dNTP Set (100 mM)?
✅ Ultra-pure nucleotides for high-sensitivity applications
✅ Individually bottled for flexible PCR setup
✅ Proven stability under demanding lab conditions
✅ Ideal for custom PCR protocols and advanced molecular biology workflows
✅ Available in multiple pack sizes to match your throughput needs
This set is ideal for any researcher performing PCR, cloning, sequencing, or high-fidelity DNA synthesis, and requiring reliable, high-performance reagents to ensure reproducible results.
- Pack Size: 4 x 0.25 mL 4 x 1 mL 4 x 5 mL