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Thermo Scientific™ Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent
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Specifications:
| Application | Capillary Electrophoresis | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent provides a simple, one-step enzymatic solution for removing excess primers and unincorporated nucleotides from PCR products. Designed for Sanger sequencing and SNP analysis, it combines exonuclease I and shrimp alkaline phosphatase (SAP) enzymes to digest residual primers and dephosphorylate free dNTPs in a single pipetting step—eliminating the need for columns, beads, or filtration methods.
With high recovery rates and minimal sample handling, ExoSAP-IT™ reduces the risk of cross-contamination while preserving the integrity of your amplicons for high-accuracy downstream analysis.
Key Features
- Removes residual primers and dNTPs enzymatically in one step
- High sample recovery—up to 100% PCR product retention
- Compatible with Sanger sequencing and SNP genotyping workflows
- Eliminates need for column-based cleanup and sample transfer
- Simple reaction: one pipetting step followed by two incubations
- Enzyme inactivation step ensures compatibility with downstream applications
Specifications
| Property | Detail |
|---|---|
| Product Type | PCR Product Cleanup Reagent (Enzymatic) |
| Enzyme Composition | Recombinant Exonuclease I + Shrimp Alkaline Phosphatase (SAP) |
| Protein Form | Recombinant |
| Format | Ready-to-use liquid formulation |
| Sample Type | PCR Products |
| Applications | PCR Purification, DNA Sequencing, SNP Analysis |
| No. of Reactions | 100 to 5000 reactions (varies by catalog number) |
| Compatible Workflows | Sanger Sequencing, SNP Genotyping |
| Storage Conditions | –20 °C |
| Shipping Condition | Dry Ice |
| Regulatory Status | For Research Use Only; not for diagnostic procedures |
Packaging Options
| Catalog # | No. of Reactions | Volume | Description |
|---|---|---|---|
| 78200.200.UL | 100 Reactions | — | Entry-level usage |
| 78201.1.ML | 500 Reactions | 1 mL | Mid-scale cleanup reagent |
| 78202.4X.1.ML | 2,000 Reactions | 4 × 1 mL | High-throughput applications |
| 78205.10.ML | 5,000 Reactions | 10 mL | Bulk cleanup volume |
Applications
- Enzymatic cleanup of PCR amplicons
- Primer and nucleotide removal before Sanger sequencing
- Sample preparation for SNP genotyping
- Post-PCR purification in high-throughput workflows
- Genetic mutation detection and sequence verification
The ExoSAP-IT™ PCR Product Cleanup Reagent offers a highly efficient, scalable, and easy-to-use enzymatic purification method for preparing PCR products for sequencing and genotyping. Eliminating the need for multi-step column purifications, it minimizes sample loss, saves time, and ensures compatibility with downstream detection technologies. Ideal for molecular biology laboratories that require clean, high-quality templates for accurate DNA sequencing and mutation analysis.
- Reactions: 100 rxn 500 rxn 2000 rxn 5000 Rxns