Thermo Scientific™ 5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) ≥99%

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Specifications:
Application Colorimetric Assay
Storage Temperature Ambient
Product Type Laboratory Chemical Forms Powder
Product Brand Thermo Fisher Scientific™, thermo Scientific
Product Grade Analytical grade Formula C₁₄H₈N₂O₈S₂

5,5'-Dithiobis(2-nitrobenzoic acid), commonly known as Ellman’s Reagent or DTNB, is a widely used compound in biochemistry and molecular biology for the quantitative determination of free sulfhydryl (–SH) groups in proteins and peptides. It reacts with thiol groups to form a mixed disulfide and releases 2-nitro-5-thiobenzoate (TNB), a yellow-colored product with a characteristic absorbance at 412 nm, making it ideal for spectrophotometric analysis.

Supplied at ≥99% purity, this reagent appears as a yellow crystalline powder and is stable under ambient conditions. It is a gold standard reagent for thiol quantification, enzyme activity assays, and protein modification studies.

Key Features

  • High purity: ≥99%
  • Spectrophotometric detection at λ = 412 nm
  • Sensitive and specific for thiol group quantification
  • Compatible with a wide range of biological buffers
  • Ideal for use in protein, enzyme, and peptide analysis
  • Stable yellow powder for long-term storage
  • GHS07 irritant classification – handle with PPE

Applications

  • Quantification of free sulfhydryl groups in biomolecules
  • Enzyme kinetics and activity studies (e.g. glutathione reductase assays)
  • Detection of thiol-disulfide exchange reactions
  • Protein structure and stability investigations
  • Monitoring of glutathione (GSH) levels
  • Used in colorimetric assays for thiol estimation in plasma, tissues, and cell extracts

Chemical & Physical Properties

PropertyValue
Chemical Name5,5'-Dithiobis(2-nitrobenzoic acid)
SynonymsDTNB, Ellman’s reagent
CAS Number69-78-3
Molecular FormulaC₁₄H₈N₂O₈S₂
Molecular Weight396.36 g/mol
MDL NumberMFCD00007140
Melting Point~240 °C (decomposition)
AppearanceYellow crystalline powder
EINECS Number200-714-4
Storage TemperatureAmbient
Hazard ClassificationGHS07 – Irritant

Available Packaging

Catalog Number
Pack TypePack Size
A14331.03
Glass bottle (solids)1 g
A14331.06
Glass bottle (solids)5 g
A14331.14
Glass bottle (solids)25 g


Thermo Scientific™ DTNB (Ellman’s Reagent), ≥99% is an essential reagent for quantitative analysis of thiol groups in proteins, enzymes, and other biomolecules. With high purity and excellent stability, it is a reliable tool for researchers in biochemistry, enzymology, and molecular diagnostics, offering sensitive and reproducible results via simple spectrophotometric measurement.

  • Pack Size:   1g 5g 25g
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