Thermo Scientific™ 5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) ≥99%
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Specifications:
Application | Colorimetric Assay | ||
Storage Temperature | Ambient | ||
Product Type | Laboratory Chemical | Forms | Powder |
Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
Product Grade | Analytical grade | Formula | C₁₄H₈N₂O₈S₂ |
5,5'-Dithiobis(2-nitrobenzoic acid), commonly known as Ellman’s Reagent or DTNB, is a widely used compound in biochemistry and molecular biology for the quantitative determination of free sulfhydryl (–SH) groups in proteins and peptides. It reacts with thiol groups to form a mixed disulfide and releases 2-nitro-5-thiobenzoate (TNB), a yellow-colored product with a characteristic absorbance at 412 nm, making it ideal for spectrophotometric analysis.
Supplied at ≥99% purity, this reagent appears as a yellow crystalline powder and is stable under ambient conditions. It is a gold standard reagent for thiol quantification, enzyme activity assays, and protein modification studies.
Key Features
- High purity: ≥99%
- Spectrophotometric detection at λ = 412 nm
- Sensitive and specific for thiol group quantification
- Compatible with a wide range of biological buffers
- Ideal for use in protein, enzyme, and peptide analysis
- Stable yellow powder for long-term storage
- GHS07 irritant classification – handle with PPE
Applications
- Quantification of free sulfhydryl groups in biomolecules
- Enzyme kinetics and activity studies (e.g. glutathione reductase assays)
- Detection of thiol-disulfide exchange reactions
- Protein structure and stability investigations
- Monitoring of glutathione (GSH) levels
- Used in colorimetric assays for thiol estimation in plasma, tissues, and cell extracts
Chemical & Physical Properties
Property | Value |
---|---|
Chemical Name | 5,5'-Dithiobis(2-nitrobenzoic acid) |
Synonyms | DTNB, Ellman’s reagent |
CAS Number | 69-78-3 |
Molecular Formula | C₁₄H₈N₂O₈S₂ |
Molecular Weight | 396.36 g/mol |
MDL Number | MFCD00007140 |
Melting Point | ~240 °C (decomposition) |
Appearance | Yellow crystalline powder |
EINECS Number | 200-714-4 |
Storage Temperature | Ambient |
Hazard Classification | GHS07 – Irritant |
Available Packaging
Catalog Number | Pack Type | Pack Size | |
---|---|---|---|
A14331.03 | Glass bottle (solids) | 1 g | |
A14331.06 | Glass bottle (solids) | 5 g | |
A14331.14 | Glass bottle (solids) | 25 g |
Thermo Scientific™ DTNB (Ellman’s Reagent), ≥99% is an essential reagent for quantitative analysis of thiol groups in proteins, enzymes, and other biomolecules. With high purity and excellent stability, it is a reliable tool for researchers in biochemistry, enzymology, and molecular diagnostics, offering sensitive and reproducible results via simple spectrophotometric measurement.
- Pack Size: 1g 5g 25g