Thermo Scientific™ 5-Methylphenazinium methyl sulphate ≥98%
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Specifications:
Application | Colorimetric Assay | ||
Storage Temperature | Ambient | ||
Product Type | Laboratory Chemical | Forms | Powder |
Product Brand | Thermo Fisher Scientific™, ThermoPro | ||
Product Grade | Analytical grade | Formula | C₁₄H₁₄N₂O₄S |
5-Methylphenazinium methyl sulphate (PMS) is a water-soluble redox dye frequently used as an electron carrier in various biochemical assays, especially in conjunction with tetrazolium salts like MTT, INT, or XTT. It is commonly employed in NADH/NADPH-coupled dehydrogenase activity assays, where PMS transfers electrons from the reduced coenzyme to the colorimetric reagent, producing a measurable colored formazan product.
Thermo Scientific supplies PMS at ≥98% purity, with stringent specifications including UV spectrophotometry (≥97.5%), GC purity (≥96%), and a titration assay of ≥99% (on dry basis). It is delivered as a dark yellow to brown crystalline powder and requires freezer storage for optimal stability.
Key Features
- ≥98% purity (Titration ≥99%)
- Strong electron carrier in redox reactions
- Used in dehydrogenase assays and cell viability tests
- Soluble in water; lambda max at 387.5 nm
- Passes HPLC suitability and membrane filtration tests
- Stable, crystalline solid with excellent shelf life
- GHS07 – Irritant; use appropriate PPE
- Requires cold storage (freezer)
Applications
- Electron transport mediator in biochemical assays
- NADH/NADPH-dependent dehydrogenase activity detection
- Commonly used with MTT, XTT, INT and similar dyes
- Colorimetric assays for enzyme kinetics and metabolic activity
- High-sensitivity tests in microbiology, cell biology, and diagnostics
- Used in membrane filtration quality control
- HPLC suitability testing in analytical chemistry
Chemical & Physical Properties
Property | Value |
---|---|
Chemical Name | 5-Methylphenazinium Methyl Sulphate |
Synonyms | PMS, Phenazine Methosulfate |
CAS Number | 299-11-6 |
Molecular Formula | C₁₄H₁₄N₂O₄S |
Molecular Weight | 306.34 g/mol |
Melting Point | 158–160 °C |
Appearance | Dark yellow to khaki to brown crystalline powder |
UV λ max | 387.5 nm (0.01 g/L in H₂O) |
UV Absorbance at 210 nm | A ≤ 0.1 |
GC Purity | ≥96% |
Titration (Ion exchange) | ≥99% (dry substance) |
HPLC Suitability | Passes test |
Membrane Filtration Test | Passes (0.4 M solution) |
Storage Conditions | Freezer (recommended ≤ –20 °C) |
Available Packaging
Catalog Number | Pack Type | Pack Size | |
---|---|---|---|
130160010 | Glass bottle (solids) | 1 g | |
130160100 | Glass bottle (solids) | 10 g | |
130160250 | Glass bottle (solids) | 25 g |
Thermo Scientific™ 5-Methylphenazinium Methyl Sulphate (PMS), 98% is a reliable, high-purity redox mediator essential for colorimetric detection of enzymatic activity. Its role as an electron shuttle in metabolic assays makes it ideal for use in biochemical research, diagnostics, and high-throughput screening, offering precise, reproducible results in enzyme activity and cell viability studies.
- Pack Size: 1g 5g 10g 25g