Thermo Scientific™ 5-Methylphenazinium methyl sulphate ≥98%

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Specifications:
Application Colorimetric Assay
Storage Temperature Ambient
Product Type Laboratory Chemical Forms Powder
Product Brand Thermo Fisher Scientific™, ThermoPro
Product Grade Analytical grade Formula C₁₄H₁₄N₂O₄S

5-Methylphenazinium methyl sulphate (PMS) is a water-soluble redox dye frequently used as an electron carrier in various biochemical assays, especially in conjunction with tetrazolium salts like MTT, INT, or XTT. It is commonly employed in NADH/NADPH-coupled dehydrogenase activity assays, where PMS transfers electrons from the reduced coenzyme to the colorimetric reagent, producing a measurable colored formazan product.

Thermo Scientific supplies PMS at ≥98% purity, with stringent specifications including UV spectrophotometry (≥97.5%), GC purity (≥96%), and a titration assay of ≥99% (on dry basis). It is delivered as a dark yellow to brown crystalline powder and requires freezer storage for optimal stability.

Key Features

  • ≥98% purity (Titration ≥99%)
  • Strong electron carrier in redox reactions
  • Used in dehydrogenase assays and cell viability tests
  • Soluble in water; lambda max at 387.5 nm
  • Passes HPLC suitability and membrane filtration tests
  • Stable, crystalline solid with excellent shelf life
  • GHS07 – Irritant; use appropriate PPE
  • Requires cold storage (freezer)

Applications

  • Electron transport mediator in biochemical assays
  • NADH/NADPH-dependent dehydrogenase activity detection
  • Commonly used with MTT, XTT, INT and similar dyes
  • Colorimetric assays for enzyme kinetics and metabolic activity
  • High-sensitivity tests in microbiology, cell biology, and diagnostics
  • Used in membrane filtration quality control
  • HPLC suitability testing in analytical chemistry

Chemical & Physical Properties

PropertyValue
Chemical Name5-Methylphenazinium Methyl Sulphate
SynonymsPMS, Phenazine Methosulfate
CAS Number299-11-6
Molecular FormulaC₁₄H₁₄N₂O₄S
Molecular Weight306.34 g/mol
Melting Point158–160 °C
AppearanceDark yellow to khaki to brown crystalline powder
UV λ max387.5 nm (0.01 g/L in H₂O)
UV Absorbance at 210 nmA ≤ 0.1
GC Purity≥96%
Titration (Ion exchange)≥99% (dry substance)
HPLC SuitabilityPasses test
Membrane Filtration TestPasses (0.4 M solution)
Storage ConditionsFreezer (recommended ≤ –20 °C)

Available Packaging

Catalog Number
Pack TypePack Size
130160010
Glass bottle (solids)1 g
130160100
Glass bottle (solids)10 g
130160250
Glass bottle (solids)25 g


Thermo Scientific™ 5-Methylphenazinium Methyl Sulphate (PMS), 98% is a reliable, high-purity redox mediator essential for colorimetric detection of enzymatic activity. Its role as an electron shuttle in metabolic assays makes it ideal for use in biochemical research, diagnostics, and high-throughput screening, offering precise, reproducible results in enzyme activity and cell viability studies.

  • Pack Size:   1g 5g 10g 25g
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