Thermo Scientific™ 3,5-Dinitrosalicylic acid 98%

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Specifications:
Application Colorimetric Assay
Storage Temperature Ambient
Product Type Laboratory Chemical Forms Powder
Product Brand Thermo Fisher Scientific™, thermo Scientific
Product Grade Analytical grade Formula C₇H₄N₂O₇

3,5-Dinitrosalicylic acid (DNS) is a nitro-substituted derivative of salicylic acid commonly used as a colorimetric reagent for the quantitative determination of reducing sugars. When heated with a sugar solution under alkaline conditions, DNS is reduced to 3-amino-5-nitrosalicylic acid, forming a reddish-brown complex measurable by UV-Vis spectrophotometry.

With a purity of ≥98% and titration value of ≥97.5%, this yellow crystalline powder is ideal for use in enzyme assays, biochemical studies, and analytical chemistry protocols, particularly in carbohydrate quantification and food chemistry.

Key Features

  • ≥98% purity
  • Standard reagent for reducing sugar determination
  • Appears as yellow crystalline powder
  • Reliable performance in UV-Vis assays and colorimetric tests
  • Supplied in sealed glass bottles for stability
  • GHS07 classified (irritant) — use with appropriate PPE

Applications

  • Quantitative detection of reducing sugars (e.g., glucose, maltose, lactose)
  • Colorimetric reagent in DNS assay
  • Enzyme activity assays involving amylase, cellulase, and other carbohydrases
  • Food and beverage sugar content analysis
  • Biochemistry and carbohydrate metabolism research

Chemical & Physical Properties

PropertySpecification
Chemical Name3,5-Dinitrosalicylic Acid
CAS Number609-99-4
Molecular FormulaC₇H₄N₂O₇
Molecular Weight228.12 g/mol
MDL NumberMFCD00007104
EINECS Number210-204-3
AppearanceYellow powder
Melting Point170–174 °C
Purity (Titration with NaOH)≥97.5%
Hazard ClassificationGHS07 – Irritant
Storage ConditionsAmbient temperature
ADR ClassificationClass 8, Packing Group II

Available Packaging

Catalog NumberPack TypePack Size
156441000Glass bottle100 g
156445000Glass bottle500 g
156440010Glass bottle1 kg


Thermo Scientific™ 3,5-Dinitrosalicylic Acid, 98% is a high-purity reagent essential for the quantitative analysis of reducing sugars and biochemical enzyme activity assays. Its consistent performance and compatibility with standard protocols make it a trusted tool in biotechnology, food science, and analytical laboratories.

  • Pack Size:   1kg 100g 500g
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