Tacrolimus (FK506), 99.97%

Tacrolimus (FK506) is a potent immunosuppressant belonging to the macrolide lactone family. It exerts its effects by binding to FKBP12 (FK506-binding protein), forming a complex that inhibits the phosphatase activity of calcineurin, thereby preventing T-cell activation and IL-2 transcription. Tacrolimus is widely used in transplant medicine, autoimmune disease research, and neuroprotection studies.

With a purity of 99.97%, this compound is suitable for in vitro cell-based assays, biochemical studies, and in vivo animal models. It also plays a role in vascular endothelial autophagy and neuroprotection.

Key Features & Benefits

1. High-Purity Immunosuppressant for Research

• 99.97% purity ensures reliable and reproducible experimental results.
• Selectively binds FKBP12, leading to calcineurin inhibition and T-cell suppression.
• Widely used in transplantation and autoimmune disease studies.

2. Proven Mechanism in T-Cell Suppression & Inflammation Regulation

• Blocks IL-2 transcription, preventing T-cell proliferation.
• Binds immunophilins (cyclophilins & FK506-binding proteins, FKBPs), modulating immune response.
• Inhibits apoptosis and cytokine release in immune cells.

3. Versatile Research Applications (In Vitro & In Vivo Studies)

• Used in cancer immunotherapy research.
• Evaluates neuroprotective effects in ischemia-reperfusion models.
• Investigates immunosuppressive mechanisms in transplant models.

4. Ready-to-Use for Laboratory Experiments

• Provided in powder form for long-term stability (-20°C storage).
• Soluble in DMSO, ethanol, and methanol for flexible experimental use.
• Aliquot stock solutions to avoid repeated freeze-thaw cycles.

Mechanism of Action

In Vitro Studies

• Blocks nuclear translocation of cytoplasmic NF-AT (nuclear factor of activated T cells).
• Inhibits calcium-dependent T-cell activation & IL-2 production.
• Regulates immune cell apoptosis & degranulation.

In Vivo Studies

• Improves pain threshold and reduces inflammatory responses in animal models.
• Reduces oxidative stress and neuronal damage in ischemia-reperfusion injury models.
• Modulates myeloperoxidase (MPO) activity and glutathione levels in rat tissues.

Chemical & Solubility Information

Clinical & Research Applications

1. Transplantation & Autoimmune Disease Research

• Prevention of organ transplant rejection.
• Suppression of autoimmune responses in lupus, rheumatoid arthritis, and psoriasis.

2. Cancer Immunotherapy

• Investigation of calcineurin-NFAT signaling in cancer progression.
• Study of FKBP12 inhibition in tumor microenvironments.

3. Neuroprotection & Ischemia Research

• Evaluation of FK506-induced neuroprotection in stroke models.
• Potential therapeutic applications in neurodegenerative diseases (Parkinson’s, Alzheimer’s).

4. Inflammatory Disease Studies

• Modulation of cytokine expression in inflammation models.
• Assessment of Tacrolimus in chronic inflammatory diseases.

Clinical Trial Information

Storage & Handling

• Store powder at -20°C for long-term stability.
• Prepare stock solutions in DMSO, ethanol, or methanol.
• Aliquot stock solutions to avoid repeated freeze-thaw cycles.

Ordering Information

Selleckchem Tacrolimus (FK506), 99.97% is a high-purity immunosuppressant used in transplant medicine, autoimmune disease research, neuroprotection studies, and inflammation modulation. It functions by binding FKBP12, inhibiting calcineurin, and blocking IL-2 transcription, making it a valuable tool for immunology, cancer therapy, and neuroscientific research.