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Thermo Scientific™ Oxoid™ Modified Karmali Selective Supplement
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For a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42° C. Each vial for 500mL medium
Specifications:
| Application | Microbiology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Supplement | Forms | Freeze Dried |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Modified Karmali Selective Supplement (SR0205E) is a freeze-dried additive designed for the preparation of a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli. When added to Campylobacter Agar Base (Karmali) (Part No. CM0935B), the supplement enhances the selectivity of the medium by inhibiting competing flora, making it ideal for isolating Campylobacter species from faecal samples and food samples.
Key Features
- Alternative to Cycloheximide:
- Contains amphotericin B as a replacement for cycloheximide, reducing toxicity while maintaining effectiveness.
- Selective for Campylobacter:
- Enhances the isolation of Campylobacter jejuni and Campylobacter coli by suppressing competing microorganisms.
- Blood-Free Medium:
- Designed for use in blood-free formulations, reducing preparation complexity.
- Convenient and Reliable:
- Each vial supplements 500mL of medium, ensuring consistent and reproducible results.
- Wide Applicability:
- Suitable for testing faecal samples in clinical microbiology and food samples in food safety workflows.
Applications
1. Clinical Microbiology
- Isolation and identification of Campylobacter jejuni and Campylobacter coli from faecal samples for diagnosing gastrointestinal infections.
2. Food Microbiology
- Detection of Campylobacter spp. in food products, including poultry and meat, as part of food safety testing.
3. Research and Academic Studies
- Studies on Campylobacter ecology and pathogenesis in clinical and environmental samples.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Selective Supplement |
| Form | Freeze-dried |
| Description | Modified Karmali Selective Supplement |
| Applications | Clinical, food, and research microbiology |
| Compatibility | For use with Campylobacter Agar Base (Karmali) (CM0935B) |
| Quantity | 10 vials per pack |
| Yield | Each vial supplements 500mL of medium |
| Storage | 2–8°C |
Preparation Instructions
- Reconstitute: Dissolve the contents of one vial of Modified Karmali Selective Supplement in 5mL of sterile distilled water.
- Add to Base: Aseptically add the rehydrated supplement to 500mL of Campylobacter Agar Base (Karmali).
- Mix and Dispense: Thoroughly mix the medium and dispense into sterile containers.
Advantages
- Enhanced Selectivity: Effectively suppresses competing microorganisms, ensuring reliable Campylobacter isolation.
- Safer Alternative: Replaces cycloheximide with amphotericin B, reducing toxicity concerns.
- Convenient Preparation: Ready-to-use freeze-dried format simplifies preparation.
- Broad Utility: Suitable for both clinical and food microbiology applications.
- Reproducible Results: Consistent performance across multiple testing workflows.
Quality Control
| Control | Expected Results |
|---|---|
| Positive Control | Campylobacter jejuni – Good growth |
| Negative Control | Non-Campylobacter species – Suppressed |
Packaging
- Product: Modified Karmali Selective Supplement (Freeze-dried)
- Catalog Number: SR0205E
- Quantity: 10 vials per pack
- Yield: Each vial supplements 500mL of medium
The Thermo Scientific™ Oxoid™ Modified Karmali Selective Supplement (SR0205E) is an essential additive for the selective isolation of Campylobacter jejuni and Campylobacter coli. Its ability to replace cycloheximide with amphotericin B and its compatibility with Campylobacter Agar Base (Karmali) makes it ideal for clinical diagnostics, food safety testing, and microbiological research.