Invitrogen™ Novex™ AP Mouse Chemiluminescent Detection Kit

High-sensitivity alkaline phosphatase-based chemiluminescent detection for mouse primary antibodies

The Invitrogen™ Novex™ AP Mouse Chemiluminescent Detection Kit delivers ultra-sensitive, long-lasting protein detection in western blotting applications using alkaline phosphatase (AP) conjugation chemistry.

Optimized for use with nitrocellulose or PVDF membranes, the kit includes a secondary AP-conjugated anti-mouse antibody, ready-to-use CDP-Star™ chemiluminescent substrate, and an enhancer. It is designed for compatibility with most western blot protocols, with optimal performance on the iBind™ Western System.

With a straightforward workflow, the kit enables detection in less than 5 minutes, produces clean backgrounds, and provides signals that last up to 5 days, making it ideal for applications requiring re-imaging or prolonged exposure.

Key Features & Benefits

• Ultra-Sensitive Detection – Achieves high signal intensity for low-abundance proteins.
• Rapid Results – Visualize results in under 5 minutes.
• Long-Lasting Signals – Stable chemiluminescence for up to 5 days.
• High Specificity – AP-conjugated anti-mouse antibody ensures minimal background noise.
• Complete Kit – Includes secondary antibody, substrate, and enhancer for 10 mini-membranes.
• Versatile Membrane Compatibility – Works with both nitrocellulose and PVDF membranes.
• Optimized for iBind™ – Ideal for automated, hands-free western blot workflows.

Applications

• Western blot detection of proteins using mouse primary antibodies
• High-sensitivity protein expression analysis
• Quantitative and qualitative protein detection in research workflows
• Long-exposure or re-imaging western blot applications

Technical Specifications

The Invitrogen™ Novex™ AP Mouse Chemiluminescent Detection Kit provides researchers with a fast, highly sensitive, and long-lasting detection solution for western blotting with mouse primary antibodies. Offering compatibility with both nitrocellulose and PVDF membranes, and optimized for the iBind™ Western System, it ensures sharp, low-background results in minutes. Its robust signal stability and complete reagent package make it an excellent choice for high-quality, reproducible protein detection in molecular biology research.