Human Interleukin-22-binding protein (IL-22BP) Elisa Kit

The Human Interleukin-22 Binding Protein (IL-22BP) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of IL-22BP levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into immune regulation, inflammatory responses, and diseases related to IL-22 signaling pathways.

Purpose

• Quantification of IL-22BP Levels:
  • Detect and measure IL-22BP concentrations in human biological samples to study immune regulation, IL-22 signaling, and related inflammatory conditions.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique for accurate and reliable quantification of IL-22BP. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to IL-22BP.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where IL-22BP in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to IL-22BP is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing IL-22BP.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • IL-22BP concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of IL-22BP with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of IL-22BP concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Applications

• Immune Regulation Research:
  • Study IL-22BP’s role in regulating IL-22 activity and maintaining immune homeostasis.
• Inflammation Studies:
  • Investigate IL-22BP levels in inflammatory and autoimmune conditions.
• Cancer Research:
  • Explore the involvement of IL-22BP in tumor progression and immune evasion.
• Infectious Disease Research:
  • Assess IL-22BP’s role in modulating immune responses during bacterial and viral infections.
• Therapeutic Development:
  • Evaluate IL-22BP as a potential therapeutic target in immune-modulating therapies.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of IL-22BP.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated IL-22BP antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate IL-22BP concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human IL-22BP ELISA Kit is a vital tool for researchers studying immune regulation, inflammation, and diseases related to IL-22 signaling pathways. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.