Human Elastase 1(ELA1)ELISA kit

Human Elastase 1 (ELA1) ELISA Kit

The Human Elastase 1 (ELA1) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of ELA1 levels in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research involving pancreatic function, digestive health, and inflammation-related conditions.

Purpose

• Quantification of ELA1 Levels:
  • Measures ELA1 concentrations to study its role in pancreatic health, digestive processes, and inflammatory responses.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for the specific and accurate measurement of ELA1. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to ELA1.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing ELA1 in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to ELA1 is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with ELA1.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • ELA1 concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of ELA1 with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of ELA1 concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• ELA1 standards for standard curve generation.
• HRP-conjugated ELA1 detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Pancreatic Function Research:
  • Study ELA1 as a biomarker for pancreatic health and digestive enzyme activity.
• Inflammation Research:
  • Investigate ELA1’s role in inflammatory diseases and conditions.
• Digestive Health Studies:
  • Assess ELA1 in relation to exocrine pancreatic insufficiency and related disorders.
• Cancer Research:
  • Monitor ELA1 levels as a potential marker in pancreatic cancer studies.
• Drug Development:
  • Evaluate therapeutic interventions targeting pancreatic function and inflammation.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of ELA1.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated ELA1 antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate ELA1 concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Elastase 1 (ELA1) ELISA Kit is a critical tool for researchers studying pancreatic health, inflammation, and digestive enzyme activity. Its high sensitivity, specificity, and user-friendly design make it indispensable for investigating pancreatic disorders, inflammatory conditions, and related metabolic pathways.