Human Hepatitis E Virus Igm, Hev-Igm Elisa Kit, 96 Tests

The Human Hepatitis E Virus Antibody IgM (HEV-IgM) ELISA Kit is a sensitive and specific qualitative assay designed to detect HEV-IgM antibodies in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research into Hepatitis E Virus (HEV) infections, immune responses, and epidemiological studies.

Purpose

• Qualitative Determination of HEV-IgM:
  • Detect and measure HEV-IgM antibodies in human biological samples to monitor immune responses to HEV infections.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay employs a qualitative enzyme immunoassay technique for detecting HEV-IgM. The process involves:

1. Pre-Coated Plate:
   • Microplate wells are pre-coated with an antigen specific to HEV-IgM, forming a solid-phase antigen.
2. Sample Addition:
   • Samples are added to the wells, where HEV-IgM antibodies in the sample bind to the immobilized antigen.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antigen specific for HEV-IgM is added, forming an antigen-antibody-enzyme labeled antigen complex.
4. Washing Step:
   • Free and unbound components are washed away with wash buffer to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue product in wells containing HEV-IgM.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Qualitative Determination:
   • The presence of HEV-IgM is determined by comparing OD values with a CUTOFF value.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects HEV-IgM with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, and other biological fluids.
3. Qualitative Analysis:
   • Determines HEV-IgM presence based on comparison with a cutoff value.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflows.
5. Reproducibility:
   • Ensures consistent results across multiple experiments.

Kit Components

• Pre-coated Microplate with HEV-IgM-specific antigen.
• HRP-conjugated HEV antigen detection reagent.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Hepatitis E Virus Research:
  • Study immune responses to HEV infections.
• Serological Studies:
  • Assess recent or acute HEV infections through IgM detection.
• Epidemiological Studies:
  • Investigate HEV prevalence in populations.
• Clinical Diagnostics Research:
  • Support diagnostics for acute HEV infections.
• Vaccine Development:
  • Evaluate immune responses to potential HEV vaccines.

Advantages

1. High Accuracy:
   • Provides precise and reproducible detection of HEV-IgM.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add samples to the pre-coated wells.
2. Incubate with HRP-conjugated HEV antigen.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Compare OD values to the cutoff to determine HEV-IgM presence.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Hepatitis E Virus Antibody IgM (HEV-IgM) ELISA Kit is a valuable tool for detecting and measuring HEV-IgM antibodies in biological samples. Its sensitivity, specificity, and user-friendly design make it indispensable for research on HEV infections, immune response, and epidemiological studies.