Human Angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit

The Human Angiotensin II (ANG-II) ELISA Kit is a highly specific and sensitive assay designed for the quantitative measurement of ANG-II levels in human serum, plasma, culture media, or other biological fluids. This kit employs the Sandwich-ELISA technique for reliable and accurate detection of ANG-II, making it ideal for research in cardiovascular function, hypertension, and related fields.

Purpose

• Quantification of ANG-II Levels:
  • Detect and measure ANG-II concentrations in a variety of human biological samples.
• Sample Compatibility:
  • Suitable for human serum, plasma, cell culture media, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA method, as outlined below:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to ANG-II.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing ANG-II in the samples to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific for ANG-II is added to the wells to form a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background noise.
5. TMB Substrate Reaction:
   • TMB substrate solution is added, generating a blue color in the presence of HRP.
6. Stop Reaction and Measurement:
   • A stop solution is added, turning the color yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Concentration Calculation:
   • ANG-II concentration is determined by comparing the OD values of samples to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects ANG-II with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for various sample types, including serum, plasma, and culture media.
3. Quantitative Results:
   • Provides reliable measurement of ANG-II concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Demonstrates consistent results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• ANG-II standards for generating the standard curve.
• HRP-conjugated detection antibody specific for ANG-II.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Cardiovascular Research:
  • Study ANG-II's role in blood pressure regulation and vascular function.
• Hypertension Studies:
  • Investigate ANG-II as a biomarker for high blood pressure and related disorders.
• Renin-Angiotensin System (RAS) Research:
  • Explore ANG-II's impact on the RAS pathway and its influence on kidney and heart health.
• Inflammatory Response Analysis:
  • Examine ANG-II's role in inflammatory and oxidative stress pathways.
• Drug Development:
  • Assess therapeutic interventions targeting the renin-angiotensin system.

Advantages

1. High Accuracy:
   • Offers precise quantification of ANG-II with reliable standard curves.
2. Convenience:
   • Ready-to-use reagents reduce preparation time.
3. Versatility:
   • Compatible with a variety of biological samples for diverse research applications.
4. Reproducibility:
   • Ensures consistent and reliable results.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated ANG-II antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate ANG-II concentration using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles.

The Human Angiotensin II (ANG-II) ELISA Kit provides a sensitive and reliable method for measuring ANG-II levels in human biological samples. Its high specificity, broad sample compatibility, and straightforward protocol make it an essential tool for cardiovascular, hypertension, and renin-angiotensin system research.