Mouse Superoxide Dismutase, SOD ELISA Kit (Enzyme Activity)

The Human Super Oxidase Dismutase (SOD) ELISA Kit (Enzyme Activity) is a precise and reliable tool designed to measure SOD enzyme activity in various biological fluids, including human serum, plasma, culture media, and other sample types. By employing a robust Sandwich-ELISA technique, this kit delivers accurate results, enabling researchers to assess the functional activity of SOD in studies related to oxidative stress, enzymatic defense mechanisms, and related physiological processes.

Purpose:

This ELISA kit is specifically designed to:

• Quantify SOD enzyme activity in human biological samples.
• Support research in oxidative stress, antioxidant defense systems, and related metabolic pathways.
• Facilitate the analysis of SOD functionality in various experimental and clinical research settings.

Principle of the Assay:

The assay uses a Sandwich-ELISA approach to detect SOD enzyme activity:

1. Pre-Coated Microplate:
   • Each well is pre-coated with a capture antibody specific for SOD, ensuring selective binding of the enzyme.
2. Sample and Standards Addition:
   • Standards or biological samples containing SOD are added to the wells, where SOD binds to the capture antibody.
3. HRP-Conjugated Antibody Binding:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific for SOD binds to a distinct epitope on the enzyme, forming a "sandwich" complex.
4. Signal Development:
   • TMB substrate reacts with the HRP to produce a colorimetric signal that is directly proportional to the SOD enzyme activity in the sample.
5. Measurement:
   • Optical density (OD) is measured spectrophotometrically at 450 nm, and enzyme activity is calculated by comparing the OD values to a standard curve.

Kit Components:

• Pre-Coated Microelisa Stripplate: Pre-coated with anti-SOD capture antibody.
• SOD Standards: For generating a standard curve to determine enzyme activity.
• HRP-Conjugated Antibody: For specific detection of SOD.
• Sample and Standard Diluent: Ensures proper sample preparation.
• Wash Buffer: Removes unbound components during washing steps.
• TMB Substrate Solution: Reacts with HRP to develop a colorimetric signal.
• Stop Solution: Stops the enzymatic reaction for stable measurements.
• Instruction Manual: Detailed protocol for assay execution.

Applications:

The Human SOD ELISA Kit is suitable for:

1. Oxidative Stress Research:
   • Evaluating SOD activity as a marker of antioxidant defense mechanisms in oxidative stress conditions.
2. Enzymatic Activity Studies:
   • Monitoring SOD activity in physiological and pathological processes.
3. Clinical Research:
   • Investigating the role of SOD in diseases linked to oxidative stress, including neurodegenerative disorders, cardiovascular diseases, and diabetes.
4. Toxicology Studies:
   • Assessing the effects of environmental toxins, chemicals, or drugs on SOD enzyme activity.
5. Pharmacological Research:
   • Evaluating the impact of therapeutic agents on SOD activity and antioxidant pathways.

Key Features and Benefits:

1. Specific Measurement of Enzyme Activity:
   • Detects and quantifies active SOD enzyme, ensuring functional analysis.
2. Wide Sample Compatibility:
   • Suitable for human serum, plasma, culture media, and other biological fluids.
3. High Sensitivity and Accuracy:
   • Provides reproducible results with minimal cross-reactivity.
4. Streamlined Workflow:
   • Pre-coated microplates and ready-to-use reagents simplify the assay process.
5. Quantitative Results:
   • Enables precise determination of SOD activity through a standard curve.

Performance Characteristics:

• Detection Method: Colorimetric (450 nm OD measurement).
• Dynamic Range: Dependent on the standards provided in the kit.
• Sample Volume Required: Small volumes required for efficient analysis.
• Reproducibility: Consistent results across replicates and lots.

Storage Conditions:

• Store all components as per the manufacturer’s instructions, typically at 2–8°C for optimal stability.

Recommended Workflow:

1. Sample Preparation:
   • Collect and prepare human biological samples (e.g., serum, plasma, or culture media). Dilute if necessary.
2. Assay Setup:
   • Add standards and samples to the pre-coated wells.
3. Incubation and Washing:
   • Incubate with the HRP-conjugated antibody and perform washing steps to remove unbound components.
4. Signal Development:
   • Add TMB substrate, incubate, and stop the reaction.
5. Measurement:
   • Measure OD at 450 nm and determine enzyme activity using the standard curve.

Why Choose This Kit?

1. Validated for Enzyme Activity Measurement:
   • Specifically designed to measure active SOD, ensuring functional analysis.
2. Ease of Use:
   • Ready-to-use reagents and detailed instructions streamline the process.
3. Wide Applications:
   • Suitable for research in oxidative stress, enzymatic defense mechanisms, and disease-related studies.
4. High Sensitivity and Specificity:
   • Detects low levels of active SOD enzyme in various biological fluids.

The Human Super Oxidase Dismutase (SOD) ELISA Kit (Enzyme Activity) is a powerful and versatile tool for researchers studying antioxidant defense systems, oxidative stress, and related physiological or pathological processes. With its high sensitivity, reproducibility, and user-friendly design, this kit supports a broad range of applications in basic research, toxicology, and pharmacology.