Human total Prostate S Pecific Antigen (tPSA) ELISA Kit

The Human Total Prostate-Specific Antigen (tPSA) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of total PSA (tPSA) levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into prostate health, cancer diagnostics, and related conditions.

Purpose

• Quantification of tPSA Levels:
  • Detect and measure total PSA concentrations in human biological samples to study prostate health and cancer biomarkers.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique to ensure accurate and reliable quantification of tPSA. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to tPSA.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where tPSA in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to tPSA is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing tPSA.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • tPSA concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of tPSA with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of tPSA concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• tPSA standards for standard curve generation.
• HRP-conjugated tPSA detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Prostate Health Research:
  • Study tPSA as a biomarker for prostate function and health.
• Cancer Research:
  • Investigate tPSA levels in prostate cancer diagnosis and progression.
• Clinical Diagnostics Research:
  • Assess the ratio of free PSA to total PSA (fPSA/tPSA) as a diagnostic tool for prostate cancer differentiation.
• Therapeutic Development:
  • Monitor tPSA levels during treatment for prostate cancer or other prostate disorders.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of tPSA.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated tPSA antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate tPSA concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Total Prostate-Specific Antigen (tPSA) ELISA Kit is a critical tool for researchers studying prostate health, cancer biomarkers, and related conditions. Its precision, user-friendly design, and broad compatibility make it indispensable for clinical and experimental applications.