Human Thyroid Stimulating Hormone - Tsh Elisa Kit, 96 Tests In 96 Well Plate

The Human Thyroid Stimulating Hormone (TSH) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of TSH levels in human serum, plasma, urine, culture media, and other biological fluids. This kit is ideal for research into thyroid function, endocrine health, and related disorders.

Purpose

• Quantification of TSH Levels:
  • Detect and measure TSH concentrations in human biological samples for research into thyroid function and hormone regulation.
• Sample Compatibility:
  • Suitable for serum, plasma, urine, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique to ensure accuracy and specificity. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to TSH.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where TSH in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to TSH is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing TSH.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • TSH concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of TSH with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, urine, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of TSH concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• TSH standards for standard curve generation.
• HRP-conjugated TSH detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Thyroid Function Research:
  • Study TSH’s role in thyroid hormone regulation and metabolism.
• Endocrine Studies:
  • Investigate TSH levels in hypothyroidism, hyperthyroidism, and subclinical thyroid dysfunctions.
• Clinical Diagnostics Research:
  • Assess TSH as a biomarker for thyroid disorders and hormone imbalances.
• Reproductive Health Research:
  • Explore the relationship between TSH and fertility or pregnancy.
• Therapeutic Development:
  • Evaluate treatments targeting thyroid function and hormone replacement therapies.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of TSH.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated TSH antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate TSH concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Thyroid Stimulating Hormone (TSH) ELISA Kit is a vital tool for researchers investigating thyroid health, hormone regulation, and endocrine disorders. Its high sensitivity, specificity, and user-friendly design make it indispensable for clinical and experimental applications.