Human Anti-Hepatitis A Virus Igm Antibody, Anti-Hav Igm Elisa Kit, 96 Tests

The Human Anti-Hepatitis A Virus IgM (Anti-HAV IgM) ELISA Kit is a precise and sensitive tool for detecting IgM antibodies specific to Hepatitis A Virus (HAV) in a variety of biological samples. This kit is particularly useful for monitoring acute Hepatitis A infections and supporting research into immunological responses against HAV.

Key Features

1. Specific Detection:
   • Detects IgM antibodies against HAV, enabling differentiation of acute infections.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, urine, cerebrospinal fluid, pleuroperitoneal fluid, cell culture supernatants, and tissue samples.
3. High Sensitivity:
   • Accurately identifies even low levels of Anti-HAV IgM in biological samples.
4. User-Friendly Protocol:
   • Includes detailed instructions for sample preparation and assay performance.
5. Long-Term Storage:
   • Samples can be stored at -20°C for future analysis, ensuring flexibility in experimental timelines.

Sample Preparation

1. Serum Preparation:

• Allow blood to clot at room temperature for 10–20 minutes.
• Centrifuge at 2,000–3,000 rpm for 20 minutes to separate serum.
• If precipitates form during storage, centrifuge again before use.

2. Plasma Preparation:

• Collect whole blood in tubes with EDTA or citrate anticoagulants.
• Incubate at room temperature for 10–20 minutes.
• Centrifuge at 2,000–3,000 rpm for 20 minutes and collect plasma supernatant.
• Re-centrifuge if precipitates are present.

3. Urine Samples:

• Collect urine in aseptic tubes and centrifuge at 2,000–3,000 rpm for 20 minutes.
• Use the clear supernatant for analysis.

4. Cell Samples:

• For secretions, centrifuge cell culture supernatants at 2,000–3,000 rpm for 20 minutes and collect supernatant.
• For intracellular components, dilute cells to 1×10⁷/mL in PBS (pH 7.2–7.4), disrupt cells by freeze-thaw cycles, and centrifuge as above.

5. Tissue Samples:

• Cut, weigh, and freeze tissue samples in liquid nitrogen. Store at -80°C until use.
• Homogenize tissue in PBS (pH 7.4) at 4°C.
• Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

Important Notes

1. Timely Processing:
   • Perform sample extraction and ELISA as soon as possible after collection.
   • Avoid repeated freeze-thaw cycles to maintain sample integrity.
2. Avoid Sodium Azide:
   • NaN₃ inhibits the activity of HRP and is not compatible with this ELISA kit.

Applications

• Clinical Diagnostics:
  • Detect acute Hepatitis A Virus infections by measuring Anti-HAV IgM levels.
• Epidemiological Studies:
  • Monitor HAV exposure and prevalence in populations.
• Immunological Research:
  • Investigate immune responses to HAV during acute infection phases.
• Vaccine Evaluation:
  • Assess vaccine efficacy by detecting Anti-HAV IgM antibodies.

Advantages

1. High Specificity:
   • Exclusively detects IgM antibodies to HAV with minimal cross-reactivity.
2. Versatility:
   • Suitable for multiple sample types, including serum, plasma, and tissue homogenates.
3. Ease of Use:
   • Includes a straightforward protocol for sample preparation and assay execution.
4. Reliable Storage:
   • Samples can be stored at -20°C for extended periods, maintaining flexibility.

The Human Anti-Hepatitis A Virus IgM (Anti-HAV IgM) ELISA Kit offers a reliable and effective solution for detecting acute HAV infections. Its specificity, sensitivity, and compatibility with various biological samples make it a valuable tool for research and clinical diagnostics in virology and immunology.