Sigma-Aldrich TLC plates, Silica gel 60 F₂₅₄, Glass

Sigma-Aldrich TLC plates with Silica Gel 60 F₂₅₄ are high-performance thin-layer chromatography (TLC) plates designed for separation and identification of organic compounds, steroids, and other lipophilic substances. These plates feature a fluorescent indicator (F₂₅₄) and are supported by a glass base to ensure robustness during handling and chromatography.

Key Features:

1. Silica Gel 60 Coating:
   • Medium pore diameter: 60 Å.
   • BET specific surface area: 480–540 m²/g.
   • Pore volume (N₂ isotherm): 0.74–0.84 mL/g.
2. Fluorescent Indicator:
   • F₂₅₄ fluorescent indicator provides UV visualization at 254 nm.
   • Suitable for detecting separated compounds under UV light.
3. Precision Layer Coating:
   • Layer thickness: 210–270 µm.
   • Deviation of layer thickness: ≤35 µm ensures high reproducibility.
4. Particle Size:
   • Mean particle size: 9.7–11.7 µm.
5. Chromatographic Testing Results:
   • Colour Test (Lipophile):
     • Blue Viforganol hRf: 11–25.
     • Ceres Black G hRf: 34–48.
     • Ceres Violet Brn hRf: 52–67.
   • Steroid Test Results:
     • Hydrocortisone hRf: 25–37.
     • Reichstein S hRf: 37–49.
     • Methyltestosterone hRf: 42–54.
6. Eluents Used:
   • A: Toluene (45% relative humidity).
   • B: Ethyl acetate/Toluene (95+5 v/v; 20% relative humidity).

Available Formats:

Applications:

1. Organic Compound Separation:
   • Ideal for separating lipophilic substances, organic dyes, and steroids.
2. Steroid Testing:
   • Precise testing and identification of hydrocortisone, Reichstein S, and methyltestosterone.
3. Research and Development:
   • Extensively used in pharmaceutical, chemical, and biochemical research for compound identification and purification.
4. Analytical Chemistry:
   • Quantitative and qualitative analyses of complex mixtures.
5. Food and Beverage Analysis:
   • Used for detecting additives and contaminants.

Benefits:

• High Reproducibility: Uniform coating ensures consistent results.
• Wide Application: Supports both routine and advanced chromatographic techniques.
• Ease of Visualization: Fluorescent indicator enables clear UV detection.
• Durable Design: Glass support enhances the structural rigidity of the plate.
• Customizable Formats: Available in various dimensions and quantities for diverse laboratory needs.

Industries Served:

• Pharmaceutical Industry: Drug development and quality control.
• Chemical Industry: Research and development.
• Academic Research: Analytical training and compound testing.
• Food and Beverage Industry: Adulteration and purity analysis.
• Clinical Research: Steroid and hormone analysis.

These TLC plates are widely recognized for their superior chromatographic performance and are a preferred choice for labs conducting thin-layer chromatography for precise compound separations.