Sigma-Aldrich REDTaq® ReadyMix™ PCR Reaction Mix

General description

REDTaq® ReadyMix™ is a ready-to-use mixture of Taq DNA polymerase, 99% pure DNTPs, reaction buffer, and an inert red dye in a 2X concentrate. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. The red loading dye migrates at approximately the same rate as a 125 base pair fragment in a 1% agarose gel. The inert dye has no effect on the amplification process, and therefore, a sample can be easily re-amplified such as in “nested PCR”.

Application

REDTaq® ReadyMix™ PCR Reaction Mix has been used:

• for routine polymerase chain reaction (PCR) amplification.[1][2][3][4]
• for the PCR amplification of cDNA[5]
• in semi-quantitative PCR[6]

Features and Benefits

The benefits of REDTaq® with the convenience of ReadyMix™

• Saves preparation time
• Reduces risk of contamination from multiple pipet steps and and increased reproducibility.
• Provides consistent reaction-to-reaction performance
• No loading buffer/tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification
• The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue)
• Amplifies targets up to 7 kb in length
• Because no loading buffer has been added, a sample can be easily re-amplifed such as in nested PCR.
• Stable at 4°C or −20°C.
• Ready-mix format reduces setup time.

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL

Preparation Note

Add 25 μl of ReadyMix to template, primers and water to a final reaction volume of 50 μl. The PCR product is easily separated from the dye by standard purification methodologies. The inert red dye has no effect on automated sequencing and is suitable for restriction enzyme digestion, ligation and other downstream manipulations.