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Sigma-Aldrich HEPES ≥99% dry basis (titration), 1M solution, amniosulfonic acid buffer, ULTROL® Grade
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | Formula | C₈H₁₈N₂O₄S |
High-Purity Zwitterionic Buffer for Biological and Biochemical Applications
Sigma-Aldrich HEPES (1 M), ULTROL® Grade is a sterile-filtered, aqueous solution of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, a zwitterionic N-substituted aminosulfonic acid buffer widely used in molecular biology, cell culture, and protein chemistry. With a certified assay of ≥99% on a dry basis (titration), this 1 M HEPES solution is ideal for sensitive applications requiring low UV absorbance, precise pH control, and high reproducibility.
Key Features
- Ready-to-Use 1 M Solution: Eliminates weighing and dissolving steps
- ULTROL® Grade Quality: High-purity, low absorbance formulation suitable for demanding research
- Zwitterionic Buffer: Effective in the physiological pH range of 6.8 to 8.2
- Stable and Sterile: Sterile-filtered and stable through freezing and ambient shipping
- Low UV Absorbance: <0.05 at 260 nm (1 M in water), ideal for UV-sensitive applications
Applications
- pH Stabilization in Biological Assays
- Buffer Preparation for Cell Culture Media
- Protein Purification and Chromatography Buffers
- Electrophoresis and Nucleic Acid Handling
- Plant Physiology and Tissue Culture
Specifications
| Property | Value |
|---|---|
| Product Name | HEPES, ULTROL® Grade |
| Form | 1 M aqueous solution |
| Assay | ≥99% dry basis (titration) |
| Buffer Type | Zwitterionic N-substituted aminosulfonic acid |
| pKa (25°C) | 7.5 |
| Useful pH Range | 6.8 – 8.2 |
| Absorbance at 260 nm (1 M) | <0.05 |
| Color | Clear, colorless |
| Sterility | Sterile-filtered |
| Storage | 15–25°C; OK to freeze |
| Shipped In | Ambient |
| Manufacturer/Tradename | Calbiochem® (Merck USA Index 144654) |
| Molecular Formula | C₈H₁₈N₂O₄S |
| Molecular Weight | 238.30 g/mol |
| CAS Number | 7365-45-9 |
| MDL Number | MFCD00006158 |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| SMILES | OCCN1CCN(CC1)CCS(O)(=O)=O |
Ordering Information
| Pack Size | Catalog Number |
| 100 mL | 375368-100ML |
| 500 mL | 375368-500ML |
Sigma-Aldrich HEPES (1 M), ULTROL® Grade offers researchers a highly pure, reliable buffer system for precision work in biochemical and molecular biology workflows. Its zwitterionic nature, high pKa stability, and low UV absorbance make it a buffer of choice for applications requiring tight control of pH and sample integrity.
- Pack Size: 100 mL 500 mL