Sigma-Aldrich Ampicillin sodium salt, BioXtra, suitable for cell culture

Broad-Spectrum β-Lactam Antibiotic for Microbiology & Cell Culture Applications

Sigma-Aldrich Ampicillin Sodium Salt (BioXtra, Cell Culture Grade) is a high-purity, broad-spectrum β-lactam antibiotic used in microbiology, biotechnology, and cell culture applications. Ampicillin effectively inhibits bacterial cell wall synthesis, making it a critical antibiotic for selective bacterial growth in research and industrial applications.

This cell culture-grade Ampicillin is endotoxin-tested, ensuring its suitability for mammalian cell culture, bacterial transformation, and antibiotic resistance studies.

Key Features & Benefits

✅ Broad-Spectrum Antibiotic – Effective against both Gram-positive and Gram-negative bacteria

✅ Cell Culture-Grade (BioXtra Quality) – Suitable for bacterial selection and mammalian cell research

✅ Mode of Action – Inhibits bacterial cell wall synthesis by inactivating transpeptidases

✅ High Stability – Maintains ≥90% activity at 2-8°C and pH 3.8–5

✅ Easy Preparation – Soluble in dilute acids or bases, requires filtration for sterilization

✅ Reliable Performance – Tested for endotoxins, ensuring minimal contamination risk

Technical Specifications

Antibiotic Mode of Action & Resistance

Mode of Action:

• Ampicillin is a β-lactam antibiotic that inhibits bacterial cell-wall synthesis by targeting transpeptidases (penicillin-binding proteins) within the inner bacterial membrane.
• This disruption leads to cell wall weakening, lysis, and bacterial death, making it effective against both Gram-positive and Gram-negative bacteria.

Mode of Resistance:

• Bacteria with β-lactamase enzymes can hydrolyze the β-lactam ring of Ampicillin, rendering it inactive.
• Co-administration with β-lactamase inhibitors (e.g., clavulanic acid) can prevent this inactivation.

Antimicrobial Spectrum:

✔ Gram-Positive Bacteria – Similar to Benzylpenicillin

✔ Gram-Negative Bacteria – Similar to Tetracyclines & Chloramphenicol

Applications

🔬 Microbiology & Bacterial Culture – Selects for ampicillin-resistant bacteria in transformation assays

🦠 Mammalian Cell Culture Studies – Prevents bacterial contamination in cell cultures

⚗️ Protein Expression in Bacteria – Used for plasmid-based selection in E. coli expression systems

🧪 Pharmaceutical & Antibiotic Research – Supports drug resistance studies & antibiotic development

🏭 Food Safety & Biochemical Testing – Used in pathogen detection assays

Preparation & Usage Guidelines

1️⃣ Preparation of Ampicillin Stock Solution

• Dissolve Ampicillin Sodium Salt in sterile water or dilute acid/base solutions.
• Recommended stock concentration: 50 mg/mL.
• Filter sterilization required (do not autoclave).
• Store at -20°C for long-term stability.

2️⃣ Recommended Working Concentrations

• Bacterial Selection: 50–100 µg/mL
• Mammalian Cell Culture Protection: 10–50 µg/mL

3️⃣ Storage & Handling

• Store at 2–8°C in a dry, well-ventilated environment.
• Avoid prolonged exposure to moisture & high pH conditions.
• Keep container tightly closed when not in use.

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only. Not intended for clinical or therapeutic applications.

⚠️ Hygroscopic & Sensitive to pH Changes – Handle in a dry, controlled environment.

⚠️ Use Protective Equipment – Avoid direct contact & inhalation during handling.

Why Choose Sigma-Aldrich Ampicillin Sodium Salt (BioXtra)?

🔹 High-Purity BioXtra Grade (≥99%) – Ensures consistent antibiotic performance

🔹 Broad-Spectrum Activity Against Gram-Positive & Gram-Negative Bacteria

🔹 Cell Culture-Tested & Endotoxin-Screened – Reduces risk of contamination

🔹 Stable & Reliable Under Controlled pH Conditions

🔹 Available in Multiple Pack Sizes for Research Flexibility

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀