Santa Cruz TMPyP4 ≥90%, telomerase inhibitor

High-Purity Telomerase Inhibitor for Cancer Research & DNA Quadruplex Studies

TMPyP4 (Tetrapyridylporphyrin) is a highly potent inhibitor of human telomerase (IC₅₀ = 6.5 µM) that selectively binds to G-quadruplex DNA, disrupting telomerase function and inducing genomic instability in cancer cells. This compound has been widely used in oncology research, DNA structural studies, and telomerase-targeted drug discovery.

Available Sizes & Catalog Numbers

Key Features & Benefits

✅ Potent Telomerase Inhibitor

• IC₅₀ = 6.5 µM for human telomerase inhibition.
• Selectively stabilizes G-quadruplex DNA, blocking telomerase activity.

✅ High Purity & Quality Assurance

• ≥90% purity (TLC Assay) ensures consistent biological activity.
• Manufactured under strict quality control (Calbiochem® brand).

✅ Research-Grade Compound for Cancer & DNA Studies

• Fluoresces intensely in the presence of G-quadruplex DNA.
• Used in oncology, molecular biology, and nucleic acid research.

✅ Stable & Easy to Store

• Storage temperature: 2-8°C (OK to freeze).
• Hygroscopic and light-sensitive, stored desiccated & protected from light.

✅ Water-Soluble for Easy Preparation

• Solubility: 1 mg/mL in water, allowing easy stock solution preparation.

Technical Specifications

Biochemical & Physiological Actions

• Selective Telomerase Inhibitor – TMPyP4 binds to G-quadruplex structures, preventing telomerase from elongating telomeres.
• Induces DNA Structural Changes – Fluoresces upon binding to quadruplex DNA, making it useful for visualization and tracking in live-cell studies.
• Potential Anticancer Agent – Used in preclinical studies to evaluate telomerase-targeted cancer therapies.

IC₅₀ Target: 6.5 µM for human telomerase inhibition

Cell Permeability: No

Toxicity: Standard Handling Required

Use Cases & Applications

1. Cancer Research & Telomerase Inhibition Studies

Primary Use:

• Investigates telomerase inhibition mechanisms in cancer cells.
• Evaluates G-quadruplex stabilization as a strategy for anticancer therapy.

Practical Example:

A cancer research team uses TMPyP4 to test its effects on telomerase-positive tumor cells, analyzing changes in telomere length and cell viability.

2. DNA Quadruplex Binding Studies

Primary Use:

• Studies G-quadruplex DNA structures in genomic regulation.
• Used as a quadruplex-binding fluorescent probe in molecular biology.

Practical Example:

A structural biology lab investigates the role of quadruplex DNA in gene expression by treating cancer cells with TMPyP4 and monitoring telomere instability.

3. Drug Discovery & Anti-Telomerase Compound Screening

Primary Use:

• Used in high-throughput screening for novel telomerase inhibitors.
• Serves as a positive control in telomerase-targeted drug testing.

Practical Example:

A pharmaceutical R&D team evaluates new G-quadruplex stabilizing drugs using TMPyP4 as a reference compound, measuring its effects on DNA structures and cell cycle progression.

4. Fluorescence Microscopy & DNA Staining

Primary Use:

• Enhances visualization of G-quadruplex DNA in live cells.
• Used in fluorescence-based detection assays.

Practical Example:

A molecular imaging lab uses TMPyP4 to fluorescently label quadruplex DNA, tracking telomere localization in live-cell experiments.

Why Choose Santa Cruz TMPyP4?

🧪 High-Purity Telomerase Inhibitor – ≥90% (TLC) purity ensures reliable and reproducible results.

🔬 Fluorescent Quadruplex Binder – Highly selective binding to G-quadruplex DNA for biochemical and imaging applications.

⚡ Proven in Cancer Research – Cited in multiple peer-reviewed publications on telomerase inhibition.

🛡 Stable & Easy to Handle – Water-soluble, desiccated, and light-protected for long-term storage.

📊 Multiple Pack Sizes Available – Choose from 25 mg, 250 mg, or 500 mg quantities.

Santa Cruz TMPyP4 is a potent and selective telomerase inhibitor that binds G-quadruplex DNA, making it a valuable research tool for cancer biology, drug discovery, and DNA structural studies. Its fluorescent properties, high purity, and strong inhibitory activity (IC₅₀ = 6.5 µM) make it ideal for labs studying telomerase function, quadruplex stability, and anticancer mechanisms.